EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.12.7.2 | 0.017 | - |
NADP+ | - |
Pyrococcus furiosus | |
1.12.7.2 | 0.07 | - |
NADPH | - |
Pyrococcus furiosus | |
1.12.7.2 | 0.07 | - |
NADH | - |
Pyrococcus furiosus | |
1.12.7.2 | 0.125 | - |
NAD+ | - |
Pyrococcus furiosus | |
1.12.7.2 | 0.2 | - |
sulfur | - |
Pyrococcus furiosus | |
1.12.7.2 | 1 | - |
methyl viologen | at 120 kPa H2 | Pyrococcus furiosus | |
1.12.7.2 | 1.25 | - |
reduced methyl viologen | - |
Pyrococcus furiosus | |
1.12.99.6 | additional information | - |
additional information | Km for various electron donors | Pyrococcus furiosus | |
1.12.99.6 | 0.128 | - |
NAD+ | - |
Pyrococcus furiosus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.12.1.5 | cytoplasm | - |
Pyrococcus furiosus | 5737 | - |
1.12.7.2 | cytoplasm | - |
Pyrococcus furiosus | 5737 | - |
1.12.98.4 | cytoplasm | - |
Pyrococcus furiosus | 5737 | - |
1.12.99.6 | cytoplasm | - |
Pyrococcus furiosus | 5737 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.12.1.5 | Fe2+ | contains 21 atoms iron per mol enzyme | Pyrococcus furiosus | |
1.12.1.5 | Ni2+ | contains 0.9 atoms nickel per mol enzyme | Pyrococcus furiosus | |
1.12.7.2 | Fe | five [4Fe4S] clusters, 2 in the beta subunit and 3 in the delta subunit, additionally one [2Fe2S] cluster in the gamma subunit | Pyrococcus furiosus | |
1.12.7.2 | Fe | 21 atoms per mol heterotetramer | Pyrococcus furiosus | |
1.12.7.2 | Ni | 0.9 atoms per mol heterotetramer, NiFe-catalytic site in the alpha-subunit | Pyrococcus furiosus | |
1.12.98.4 | Fe2+ | contains 21 atoms iron per mol enzyme | Pyrococcus furiosus | |
1.12.98.4 | Ni2+ | contains 0.9 atoms nickel per mol enzyme | Pyrococcus furiosus | |
1.12.99.6 | Fe | 21 atoms per mol enzyme, 5 4Fe-4S cluster and 1 2Fe-2S cluster | Pyrococcus furiosus | |
1.12.99.6 | Ni | 0.9 atoms per mol enzyme | Pyrococcus furiosus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.12.1.5 | 24000 | - |
1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE | Pyrococcus furiosus |
1.12.1.5 | 30000 | - |
1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE | Pyrococcus furiosus |
1.12.1.5 | 39000 | - |
1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE | Pyrococcus furiosus |
1.12.1.5 | 52000 | - |
1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE | Pyrococcus furiosus |
1.12.1.5 | 320000 | - |
gel filtration | Pyrococcus furiosus |
1.12.7.2 | 24000 | - |
alpha, beta, gamma, delta, 2 * 52000 + 2 * 39000 + 2 * 30000 + 2 * 24000, SDS-PAGE | Pyrococcus furiosus |
1.12.7.2 | 30000 | - |
alpha, beta, gamma, delta, 2 * 52000 + 2 * 39000 + 2 * 30000 + 2 * 24000, SDS-PAGE | Pyrococcus furiosus |
1.12.7.2 | 39000 | - |
alpha, beta, gamma, delta, 2 * 52000 + 2 * 39000 + 2 * 30000 + 2 * 24000, SDS-PAGE | Pyrococcus furiosus |
1.12.7.2 | 52000 | - |
alpha, beta, gamma, delta, 2 * 52000 + 2 * 39000 + 2 * 30000 + 2 * 24000, SDS-PAGE | Pyrococcus furiosus |
1.12.7.2 | 320000 | - |
gel filtration | Pyrococcus furiosus |
1.12.98.4 | 24000 | - |
1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE | Pyrococcus furiosus |
1.12.98.4 | 30000 | - |
1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE | Pyrococcus furiosus |
1.12.98.4 | 39000 | - |
1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE | Pyrococcus furiosus |
1.12.98.4 | 52000 | - |
1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE | Pyrococcus furiosus |
1.12.98.4 | 320000 | - |
gel filtration | Pyrococcus furiosus |
1.12.99.6 | 24000 | - |
alpha,beta,gamma,delta, 1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE | Pyrococcus furiosus |
1.12.99.6 | 30000 | - |
alpha,beta,gamma,delta, 1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE | Pyrococcus furiosus |
1.12.99.6 | 39000 | - |
alpha,beta,gamma,delta, 1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE | Pyrococcus furiosus |
1.12.99.6 | 52000 | - |
alpha,beta,gamma,delta, 1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE | Pyrococcus furiosus |
1.12.99.6 | 320000 | - |
gel filtration | Pyrococcus furiosus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.12.1.5 | Pyrococcus furiosus | - |
- |
- |
1.12.1.5 | Pyrococcus furiosus | E7FHC4 | subunit alpha | - |
1.12.1.5 | Pyrococcus furiosus | E7FHF8 | subunit delta | - |
1.12.1.5 | Pyrococcus furiosus | E7FHN9 | subunit beta | - |
1.12.1.5 | Pyrococcus furiosus | E7FHW8 | subunit gamma | - |
1.12.7.2 | Pyrococcus furiosus | - |
strain DSM 3638 | - |
1.12.7.2 | Pyrococcus furiosus | - |
hydrogenase II | - |
1.12.98.4 | Pyrococcus furiosus | E7FHC4 and E7FHN9 and E7FHW8 and E7FHF8 | subunits alpha, beta, gamma and delta | - |
1.12.99.6 | Pyrococcus furiosus | - |
strain DSM 3638 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.12.1.5 | DEAE column chromatography, hydroxyapatite column chromatography, phenyl Sepharose column chromatography, and Superdex 200 gel filtration | Pyrococcus furiosus |
1.12.98.4 | DEAE column chromatography, hydroxyapatite column chromatography, phenyl Sepharose column chromatography, and Superdex 200 gel filtration | Pyrococcus furiosus |
1.12.99.6 | - |
Pyrococcus furiosus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.12.7.2 | 0.02 | - |
hydrogen production, oxidized ferredoxin as electron donor, hydrogenase II | Pyrococcus furiosus |
1.12.7.2 | 0.2 | - |
sulfur reduction, hydrogenase II | Pyrococcus furiosus |
1.12.7.2 | 0.98 | - |
hydrogen production, sodium dithionite as electron donor, hydrogenase II | Pyrococcus furiosus |
1.12.7.2 | 11 | - |
hydrogen oxidation, methyl viologen as electron acceptor, hydrogenase II | Pyrococcus furiosus |
1.12.7.2 | 40 | - |
hydrogen production, methyl viologen as electron donor, hydrogenase II | Pyrococcus furiosus |
1.12.7.2 | 131 | - |
hydrogen oxidation, benzyl viologen as electron acceptor, hydrogenase II | Pyrococcus furiosus |
1.12.99.6 | additional information | - |
specific activities with various electron donors | Pyrococcus furiosus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.12.1.5 | H2 + NAD+ | - |
Pyrococcus furiosus | H+ + NADH | - |
r | |
1.12.1.5 | H2 + NADP+ | - |
Pyrococcus furiosus | H+ + NADPH | - |
r | |
1.12.1.5 | additional information | ferredoxin from Pyrococcus furiosus is not an efficient electron carrier | Pyrococcus furiosus | ? | - |
? | |
1.12.7.2 | H+ + NADH | - |
Pyrococcus furiosus | H2 + NAD+ | - |
r | |
1.12.7.2 | H+ + reduced methyl viologen | - |
Pyrococcus furiosus | H2 + oxidized methyl viologen | - |
? | |
1.12.7.2 | H2 + electron acceptor | methyl viologen as electron acceptor | Pyrococcus furiosus | H+ + reduced electron acceptor | - |
r | |
1.12.7.2 | H2 + electron acceptor | benzyl viologen as electron acceptor | Pyrococcus furiosus | H+ + reduced electron acceptor | - |
r | |
1.12.7.2 | H2 + electron acceptor | NADP+ as electron acceptor | Pyrococcus furiosus | H+ + reduced electron acceptor | - |
r | |
1.12.7.2 | H2 + NAD+ | - |
Pyrococcus furiosus | H+ + NADH | - |
r | |
1.12.7.2 | additional information | ferredoxin is not an efficient electron carrier for both hydrogenases | Pyrococcus furiosus | ? | - |
? | |
1.12.7.2 | S + NADPH | - |
Pyrococcus furiosus | H2S + NADP+ | - |
? | |
1.12.98.4 | H2 + benzyl viologen | highest activity | Pyrococcus furiosus | H2S + reduced benzyl viologen | - |
? | |
1.12.98.4 | H2 + methyl viologen | - |
Pyrococcus furiosus | H2S + reduced methyl viologen | - |
? | |
1.12.98.4 | H2 + polysulfide(n) | - |
Pyrococcus furiosus | H2S + polysulfide(n-1) | - |
r | |
1.12.98.4 | additional information | ferredoxin from Pyrococcus furiosus is not an efficient electron carrier | Pyrococcus furiosus | ? | - |
? | |
1.12.98.4 | NADPH + H+ + polysulfide(n) | - |
Pyrococcus furiosus | NADP+ + polysulfide(n-1) | - |
r | |
1.12.99.6 | H2 + acceptor | benzyl viologen, methyl viologen, NADPH and NADH can serve as electron donors | Pyrococcus furiosus | H+ + reduced acceptor | - |
? | |
1.12.99.6 | H2 + NAD+ | - |
Pyrococcus furiosus | NADH + H+ | - |
r | |
1.12.99.6 | H2 + S | - |
Pyrococcus furiosus | H2S | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.12.1.5 | heterotetramer | 1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE | Pyrococcus furiosus |
1.12.7.2 | octamer | alpha, beta, gamma, delta, 2 * 52000 + 2 * 39000 + 2 * 30000 + 2 * 24000, SDS-PAGE | Pyrococcus furiosus |
1.12.98.4 | heterotetramer | 1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE | Pyrococcus furiosus |
1.12.99.6 | tetramer | alpha,beta,gamma,delta, 1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE | Pyrococcus furiosus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.12.1.5 | H-II | - |
Pyrococcus furiosus |
1.12.1.5 | hydrogenase II | - |
Pyrococcus furiosus |
1.12.98.4 | H-II | - |
Pyrococcus furiosus |
1.12.98.4 | hydrogenase II | - |
Pyrococcus furiosus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.12.7.2 | 90 | - |
above 90°C, hydrogenase II | Pyrococcus furiosus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.12.7.2 | 95 | - |
50% inactivation after 6 h, hydrogenase II | Pyrococcus furiosus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.12.99.6 | additional information | - |
additional information | turnover number for various electron donors | Pyrococcus furiosus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.12.1.5 | FAD | contains 0.83 FAD per mol enzyme | Pyrococcus furiosus | |
1.12.1.5 | NADPH | - |
Pyrococcus furiosus | |
1.12.1.5 | [2Fe-2S]-center | contains 1 [2Fe-2S]-center | Pyrococcus furiosus | |
1.12.1.5 | [4Fe-4S]-center | contains 2 [4Fe-4S]-centers | Pyrococcus furiosus | |
1.12.7.2 | FAD | putative nucleotide-binding site in the gamma subunit | Pyrococcus furiosus | |
1.12.7.2 | FAD | hydrogenase II, 0.83 mol per mol heterotetramer | Pyrococcus furiosus | |
1.12.7.2 | NADH | putative nucleotide-binding site in the gamma subunit | Pyrococcus furiosus | |
1.12.7.2 | NADPH | putative nucleotide-binding site in the gamma subunit | Pyrococcus furiosus | |
1.12.98.4 | FAD | contains 0.83 FAD per mol enzyme | Pyrococcus furiosus | |
1.12.98.4 | NADH | NADPH and NADH are equally efficient as electron donors for H2 production | Pyrococcus furiosus | |
1.12.98.4 | NADPH | NADPH and NADH are equally efficient as electron donors for H2 production | Pyrococcus furiosus | |
1.12.98.4 | [2Fe-2S]-center | contains 1 [2Fe-2S] center | Pyrococcus furiosus | |
1.12.98.4 | [4Fe-4S]-center | contains 2 [4Fe-4S]-centers | Pyrococcus furiosus | |
1.12.99.6 | FAD | 0.83 mol FAD per enzyme | Pyrococcus furiosus |