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Literature summary extracted from

  • Ishii, M.; Takishita, S.; Iwasaki, T.; Peerapornpisal, Y.; Yoshino, J.I.; Kodama, T.; Igarashi, Y.
    Purification and characterization of membrane-bound hydrogenase from Hydrogenobacter thermophilus strain TK-6, an obligately autotrophic, thermophilic, hydrogen-oxidizing bacterium (2000), Biosci. Biotechnol. Biochem., 64, 492-502.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.12.99.6 300
-
 oxidized methionaquinone
-
 Hydrogenobacter thermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.12.99.6 Fe 10.9 mol per tetramer, 3Fe-4S cluster Hydrogenobacter thermophilus
1.12.99.6 Ni 1.06 mol per tetramer Hydrogenobacter thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.12.99.6 38000
-
 alpha2,beta2, 2 * 60000 + 2 * 38000, SDS-PAGE Hydrogenobacter thermophilus
1.12.99.6 60000
-
 alpha2,beta2, 2 * 60000 + 2 * 38000, SDS-PAGE Hydrogenobacter thermophilus
1.12.99.6 200000
-
 gel filtration Hydrogenobacter thermophilus

Organism

EC Number Organism UniProt Comment Textmining
1.12.99.6 Hydrogenobacter thermophilus
-
-
-
1.12.99.6 Hydrogenobacter thermophilus TK-6 / IAM 12695
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.99.6 homogeneity Hydrogenobacter thermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.12.99.6 0.0613
-
-
 Hydrogenobacter thermophilus
1.12.99.6 285
-
-
 Hydrogenobacter thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.99.6 H2 + acceptor methylene blue, methyl viologen, methionaquinone, FMN and FAD can serve as electron acceptors, but not NAD+ or NADP+ Hydrogenobacter thermophilus H+ + reduced acceptor
-
 ?
1.12.99.6 H2 + acceptor methylene blue, methyl viologen, methionaquinone, FMN and FAD can serve as electron acceptors, but not NAD+ or NADP+ Hydrogenobacter thermophilus TK-6 / IAM 12695 H+ + reduced acceptor
-
 ?
1.12.99.6 H2 + methionaquinone
-
 Hydrogenobacter thermophilus methionaquinol
-
 r
1.12.99.6 H2 + methionaquinone
-
 Hydrogenobacter thermophilus TK-6 / IAM 12695 methionaquinol
-
 r
1.12.99.6 H2 + oxidized methionaquinone
-
 Hydrogenobacter thermophilus reduced methionaquinone
-
 r
1.12.99.6 H2 + oxidized methionaquinone
-
 Hydrogenobacter thermophilus TK-6 / IAM 12695 reduced methionaquinone
-
 r

Subunits

EC Number Subunits Comment Organism
1.12.99.6 tetramer alpha2,beta2, 2 * 60000 + 2 * 38000, SDS-PAGE Hydrogenobacter thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.12.99.6 80 95
-
 Hydrogenobacter thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.12.99.6 30
-
 50% activity after 20 min under air Hydrogenobacter thermophilus
1.12.99.6 70
-
 50% activity after 120 min under argon or hydrogen, 50% activity after 70 min under air Hydrogenobacter thermophilus
1.12.99.6 80
-
 50% activity after 22 min under hydrogen, 30 min under argon and 15 min under air Hydrogenobacter thermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.12.99.6 48.3
-
 methionaquinone
-
 Hydrogenobacter thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.12.99.6 8
-
-
 Hydrogenobacter thermophilus