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Literature summary extracted from
- Characterization and thermostability of a membrane-bound hydrogenase from a thermophilic hydrogen oxidizing bacterium, Bacillus schlegelii (1993), Biosci. Biotechnol. Biochem., 57, 1177-1179.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.12.99.6 | Cu2+ | complete inhibition at 1 mM | Hydrogenibacillus schlegelii |  |
| 1.12.99.6 | Hg2+ | complete inhibition at 1 mM | Hydrogenibacillus schlegelii | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.12.99.6 | Ni | NiFe-hydrogenase | Hydrogenibacillus schlegelii | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.12.99.6 | 28000 | - |
1 * 66000 + 1 * 28000, SDS-PAGE | Hydrogenibacillus schlegelii |
| 1.12.99.6 | 66000 | - |
1 * 66000 + 1 * 28000, SDS-PAGE | Hydrogenibacillus schlegelii |
| 1.12.99.6 | 100000 | - |
gel filtration | Hydrogenibacillus schlegelii |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.12.99.6 | Hydrogenibacillus schlegelii | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.12.99.6 | - |
Hydrogenibacillus schlegelii |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.12.99.6 | 0.4 | 0.7 | H2 evolution | Hydrogenibacillus schlegelii |
| 1.12.99.6 | 84 | - |
H2 oxidation | Hydrogenibacillus schlegelii |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.12.99.6 | H2 + acceptor | methylene blue acts as electron acceptor | Hydrogenibacillus schlegelii | H+ + reduced acceptor | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.12.99.6 | dimer | 1 * 66000 + 1 * 28000, SDS-PAGE | Hydrogenibacillus schlegelii |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.12.99.6 | 60 | - |
assay at | Hydrogenibacillus schlegelii |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.12.99.6 | 60 | - |
90% activity after 12 h | Hydrogenibacillus schlegelii |
| 1.12.99.6 | 70 | - |
50% activity after 4 h | Hydrogenibacillus schlegelii |
| 1.12.99.6 | 80 | - |
50% activity after 2.5 h | Hydrogenibacillus schlegelii |
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