Literature summary extracted from

Adams, M.W.W.; Johnson, M.K.; Zambrano, I.C.; Mortenson, L.E.
On the novel hydrogen-activating iron-sulfur center of the "iron-only" hydrogenases (1986), Biochimie, 68, 35-41.

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
1.12.99.6	cytoplasm	-	Clostridium pasteurianum	5737	-
1.12.99.6	cytoplasm	-	Megasphaera elsdenii	5737	-
1.12.99.6	periplasm	-	Desulfovibrio vulgaris	-	-

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
1.12.99.6	Fe	iron-sulfur cluster as well as 4Fe-4S-ferredoxin-type cluster	Clostridium pasteurianum
1.12.99.6	Fe	iron-sulfur cluster as well as 4Fe-4S-ferredoxin-type cluster	Megasphaera elsdenii
1.12.99.6	Fe	iron-sulfur cluster as well as 4Fe-4S-ferredoxin-type cluster	Desulfovibrio vulgaris

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
1.12.99.6	50000	-	SDS-PAGE	Desulfovibrio vulgaris
1.12.99.6	52000	-	SDS-PAGE	Megasphaera elsdenii
1.12.99.6	53000	-	hydrogenase II, SDS-PAGE	Clostridium pasteurianum
1.12.99.6	60000	-	hydrogenase I, SDS-PAGE	Clostridium pasteurianum

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.12.99.6	Clostridium pasteurianum	-	-	-
1.12.99.6	Desulfovibrio vulgaris	-	-	-
1.12.99.6	Desulfovibrio vulgaris Hildenborough	-	-	-
1.12.99.6	Megasphaera elsdenii	-	-	-

Oxidation Stability

EC Number	Oxidation Stability	Organism
1.12.99.6	enzyme is extremely sensitive to inactivation by O2	Clostridium pasteurianum
1.12.99.6	enzyme is extremely sensitive to inactivation by O2	Megasphaera elsdenii

Purification (Commentary)

EC Number	Purification (Comment)	Organism
1.12.99.6	-	Clostridium pasteurianum
1.12.99.6	-	Megasphaera elsdenii
1.12.99.6	-	Desulfovibrio vulgaris

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.12.99.6	H2 + acceptor	-	Desulfovibrio vulgaris	H+ + reduced acceptor	-	r
1.12.99.6	H2 + acceptor	2 different enzymes, hydrogenase I and II	Clostridium pasteurianum	H+ + reduced acceptor	-	r
1.12.99.6	H2 + acceptor	2 different enzymes, hydrogenase I and II	Megasphaera elsdenii	H+ + reduced acceptor	-	r
1.12.99.6	H2 + acceptor	-	Desulfovibrio vulgaris Hildenborough	H+ + reduced acceptor	-	r

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Release 2023.1 (January 2023)