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Literature summary extracted from

  • Que, L.; Widom, J.; Crawford, R.L.
    3,4-Dihydroxyphenylacetate 2,3-dioxygenase. A manganese(II) dioxygenase from Bacillus brevis (1981), J. Biol. Chem., 256, 10941-10944.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.15 1,10-phenanthroline
-
 Brevibacillus brevis
1.13.11.15 3,4-dihydroxybenzoate
-
 Brevibacillus brevis
1.13.11.15 3,4-dihydroxycinnamate
-
 Brevibacillus brevis
1.13.11.15 3,4-Dihydroxyphenylpropionate
-
 Brevibacillus brevis
1.13.11.15 4-methylcatechol
-
 Brevibacillus brevis
1.13.11.15 4-nitrocatechol
-
 Brevibacillus brevis
1.13.11.15 8-hydroxyquinoline
-
 Brevibacillus brevis
1.13.11.15 catechol
-
 Brevibacillus brevis
1.13.11.15 m-hydroxyphenylacetate
-
 Brevibacillus brevis
1.13.11.15 p-hydroxyphenylacetate
-
 Brevibacillus brevis
1.13.11.15 PCMB
-
 Brevibacillus brevis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.15 Iron the enzyme contains 0.04-0.2 gatom of iron Brevibacillus brevis
1.13.11.15 Manganese approximately 2 gatom of manganese per mol of protein Brevibacillus brevis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.15 36000
-
 4 * 36000, SDS-PAGE Brevibacillus brevis
1.13.11.15 120000
-
 equilibrium sedimentation Brevibacillus brevis
1.13.11.15 140000
-
 gel filtration Brevibacillus brevis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.15 3,4-dihydroxyphenylacetate + O2 Brevibacillus brevis enzyme catalyzes the estradiol cleavage of catechols 2-hydroxy-5-carboxymethylmuconate semialdehyde
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.15 Brevibacillus brevis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.15
-
 Brevibacillus brevis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.15 3,4-dihydroxymandelate + O2 14% of the activity with 3,4-dihydroxyphenylacetate Brevibacillus brevis ?
-
 ?
1.13.11.15 3,4-dihydroxyphenylacetate + O2
-
 Brevibacillus brevis 2-hydroxy-5-carboxymethylmuconate semialdehyde
-
 ?
1.13.11.15 3,4-dihydroxyphenylacetate + O2 enzyme catalyzes the estradiol cleavage of catechols Brevibacillus brevis 2-hydroxy-5-carboxymethylmuconate semialdehyde
-
 ?
1.13.11.15 3,4-dihydroxyphenylpropionate + O2 5% of the activity with 3,4-dihydroxyphenylacetate Brevibacillus brevis 2-hydroxy-5-carboxyethylmuconate semialdehyde
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.15 tetramer 4 * 36000, SDS-PAGE Brevibacillus brevis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.15 8
-
-
 Brevibacillus brevis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.13.11.15 7
-
 most stable at Brevibacillus brevis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.13.11.15 0.27
-
 m-hydroxyphenylacetate
-
 Brevibacillus brevis
1.13.11.15 0.3
-
 3,4-dihydroxycinnamate
-
 Brevibacillus brevis
1.13.11.15 0.5
-
 4-nitrocatechol
-
 Brevibacillus brevis
1.13.11.15 1
-
 3,4-Dihydroxyphenylpropionate
-
 Brevibacillus brevis
1.13.11.15 1.5
-
 4-methylcatechol
-
 Brevibacillus brevis
1.13.11.15 1.8
-
 p-hydroxyphenylacetate
-
 Brevibacillus brevis
1.13.11.15 2.3
-
 3,4-dihydroxybenzoate
-
 Brevibacillus brevis
1.13.11.15 3.8
-
 catechol
-
 Brevibacillus brevis