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Literature summary extracted from

  • Kutty, R.K.; Devi, N.A.; Veeraswamy, M.; Ramesh, S.; Rao, P.V.S.
    Degradation of (+/-)-synephrine by Arthrobacter synephrinum. Oxidation of 3,4-dihydroxyphenylacetate to 2-hydroxy-5-carboxymethyl-muconate semialdehyde (1977), Biochem. J., 167, 163-170.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
1.13.11.15 inactivation by 6 M guanidine hydrochloride Arthrobacter synephrinum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.15 1,10-phenanthroline no inhibition Arthrobacter synephrinum
1.13.11.15 5,5'-dithiobis-(2-nitrobenzoate) 1 mM, complete inhibition Arthrobacter synephrinum
1.13.11.15 8-hydroxyquinoline no inhibition Arthrobacter synephrinum
1.13.11.15 additional information no inhibition by EDTA or 1,1'-bipyridine Arthrobacter synephrinum
1.13.11.15 PCMB 1 mM, 59% inhibition Arthrobacter synephrinum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.15 0.025
-
 3,4-Dihydroxyphenylacetate
-
 Arthrobacter synephrinum
1.13.11.15 0.5
-
 3,4-dihydroxymandelate
-
 Arthrobacter synephrinum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.15 282000
-
 gel filtration Arthrobacter synephrinum

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.15 Arthrobacter synephrinum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.15 partial Arthrobacter synephrinum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.15 2.51
-
-
 Arthrobacter synephrinum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.15 3,4-dihydroxymandelate + O2 50% of the activity with 3,4-dihydroxyphenylacetate Arthrobacter synephrinum ?
-
 ?
1.13.11.15 3,4-dihydroxyphenylacetate + O2
-
 Arthrobacter synephrinum 2-hydroxy-5-carboxymethylmuconate semialdehyde
-
 ?
1.13.11.15 3,4-dihydroxyphenylpropionate + O2 4% of the activity with 3,4-dihydroxyphenylacetate Arthrobacter synephrinum 2-hydroxy-5-carboxyethylmuconate semialdehyde
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.15 50
-
-
 Arthrobacter synephrinum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.11.15 50
-
 30 min, stable Arthrobacter synephrinum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.15 7.2
-
-
 Arthrobacter synephrinum