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Literature summary extracted from

  • Kobayashi, T.; Ishida, T.; Horiike, K.; Takahara, Y.; Numao, N.; Nakazawa, A.; Nakazawa, T.; Nozaki, M.
    Overexpression of Pseudomonas putida catechol 2,3-dioxygenase with high specific activity by genetically engineered Escherichia coli (1995), J. Biochem., 117, 614-622.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.2 expression in Escherichia coli W3110 Pseudomonas putida

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.13.11.2
-
Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.2 (1R,2R)-trans-1,2-cyclohexanediol
-
Pseudomonas putida
1.13.11.2 (1S,2S)-trans-1,2-cyclohexanediol
-
Pseudomonas putida
1.13.11.2 1-naphthoquinoline
-
Pseudomonas putida
1.13.11.2 4-Methyl-2-nitrophenol
-
Pseudomonas putida
1.13.11.2 acetone
-
Pseudomonas putida
1.13.11.2 benzoate
-
Pseudomonas putida
1.13.11.2 m-fluorophenol
-
Pseudomonas putida
1.13.11.2 m-Hydroxybenzoate
-
Pseudomonas putida
1.13.11.2 m-Nitrophenol
-
Pseudomonas putida
1.13.11.2 nitrobenzene
-
Pseudomonas putida
1.13.11.2 o-aminophenol
-
Pseudomonas putida
1.13.11.2 o-fluorophenol
-
Pseudomonas putida
1.13.11.2 o-Nitrophenol
-
Pseudomonas putida
1.13.11.2 p-fluorophenol
-
Pseudomonas putida
1.13.11.2 p-nitrophenol
-
Pseudomonas putida
1.13.11.2 phenol
-
Pseudomonas putida
1.13.11.2 salicylate
-
Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.2 0.00102
-
4-Chlorocatechol
-
Pseudomonas putida
1.13.11.2 0.00129
-
4-methylcatechol
-
Pseudomonas putida
1.13.11.2 0.00164
-
3-methylcatechol
-
Pseudomonas putida
1.13.11.2 0.00187
-
catechol
-
Pseudomonas putida
1.13.11.2 0.00187
-
3,5-dichlorocatechol
-
Pseudomonas putida
1.13.11.2 0.00204
-
3-methoxycatechol
-
Pseudomonas putida
1.13.11.2 0.0228
-
Protocatechualdehyde
-
Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.2 Fe2+ 3.7 mol of iron per mol of enzyme or 0.93 mol per mol of the subunit Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.2 35000
-
4 * 35000, SDS-PAGE, 4 * 35195, MS, 4 * 35156 based on DNA sequence Pseudomonas putida
1.13.11.2 35156
-
4 * 35000, SDS-PAGE, 4 * 35195, MS, 4 * 35156 based on DNA sequence Pseudomonas putida
1.13.11.2 35195
-
4 * 35000, SDS-PAGE, 4 * 35195, MS, 4 * 35156 based on DNA sequence Pseudomonas putida
1.13.11.2 136000 144000 light scattering method Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.2 Pseudomonas putida
-
mt-2 paW1, ATCC 23973
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.2
-
Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.2 536
-
crystallized enzyme Pseudomonas putida

Storage Stability

EC Number Storage Stability Organism
1.13.11.2 0°C, 50 mM Tris-acetone buffer, pH 7.5, the inactivation rate with decreasing enzyme concentration Pseudomonas putida
1.13.11.2 4°C, Tris-acetone buffer, crystalline enzyme was stable for at most six months Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.2 3,5-dichlorocatechol + O2
-
Pseudomonas putida ?
-
?
1.13.11.2 3-methoxycatechol + O2
-
Pseudomonas putida 2-hydroxy-3-methoxy-6-oxohexa-2,4-dienoate
-
?
1.13.11.2 3-methylcatechol + O2
-
Pseudomonas putida 2-hydroxy-6-oxohepta-2,4-dienoate
-
?
1.13.11.2 4-chlorocatechol + O2
-
Pseudomonas putida 5-chloro-2-hydroxymuconic semialdehyde
-
?
1.13.11.2 4-methylcatechol + O2
-
Pseudomonas putida 2-hydroxy-3-methyl-6-oxohexa-2,4-dienoate
-
?
1.13.11.2 catechol + O2
-
Pseudomonas putida 2-hydroxymuconate semialdehyde
-
?
1.13.11.2 protocatechualdehyde + O2 weak Pseudomonas putida ?
-
?

Subunits

EC Number Subunits Comment Organism
1.13.11.2 homotetramer 4 * 35000, SDS-PAGE, 4 * 35195, MS, 4 * 35156 based on DNA sequence Pseudomonas putida

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.13.11.2 0.00056
-
1-naphthoquinoline
-
Pseudomonas putida
1.13.11.2 0.0049
-
o-aminophenol
-
Pseudomonas putida
1.13.11.2 0.0093
-
o-Nitrophenol
-
Pseudomonas putida
1.13.11.2 0.042
-
m-Nitrophenol
-
Pseudomonas putida
1.13.11.2 0.056
-
o-fluorophenol
-
Pseudomonas putida
1.13.11.2 0.111
-
p-nitrophenol
-
Pseudomonas putida
1.13.11.2 0.126
-
4-Methyl-2-nitrophenol
-
Pseudomonas putida
1.13.11.2 0.205
-
nitrobenzene
-
Pseudomonas putida
1.13.11.2 0.311
-
m-fluorophenol
-
Pseudomonas putida
1.13.11.2 0.452
-
p-fluorophenol
-
Pseudomonas putida
1.13.11.2 1.7
-
phenol
-
Pseudomonas putida
1.13.11.2 4.9
-
benzoate
-
Pseudomonas putida
1.13.11.2 5.2
-
salicylate
-
Pseudomonas putida
1.13.11.2 13
-
acetone
-
Pseudomonas putida
1.13.11.2 14.7
-
m-Hydroxybenzoate
-
Pseudomonas putida
1.13.11.2 34
-
(1R,2R)-trans-1,2-cyclohexanediol
-
Pseudomonas putida
1.13.11.2 43
-
(1S,2S)-trans-1,2-cyclohexanediol
-
Pseudomonas putida