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Literature summary extracted from

  • Murakami, S.; Nakanishi, Y.; Kodama, N.; Takenaka, S.; Shinke, R.; Aoki, K.
    Purification, characterization, and gene analysis of catechol 2,3-dioxygenase from the aniline-assimilating bacterium Pseudomonas species AW-2 (1998), Biosci. Biotechnol. Biochem., 62, 747-752.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.2 Ag+
-
 Pseudomonas sp.
1.13.11.2 CuSO4
-
 Pseudomonas sp.
1.13.11.2 Hg2+
-
 Pseudomonas sp.
1.13.11.2 o-phenanthroline not Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.2 0.001
-
 3-methylcatechol
-
 Pseudomonas sp.
1.13.11.2 0.0039
-
 catechol
-
 Pseudomonas sp.
1.13.11.2 0.0109
-
 4-methylcatechol
-
 Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.2 Fe2+ 0.6 mol of iron per mol of protein Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.2 33000
-
 4 * 33000 Pseudomonas sp.
1.13.11.2 110000
-
 gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.2 catechol + O2 Pseudomonas sp. catechol 2,3-dioxygenase is a key enzyme in the catabolism of monocyclic aromatic compounds including aniline and catalyzes the extradiol cleavage of catechol 2-hydroxymuconate semialdehyde
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.2 Pseudomonas sp.
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.2 additional information
-
-
 Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
1.13.11.2 4°C, buffer, containing 5% acetone, pH 6-7,5, 24 h, stable Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.2 3-methylcatechol + O2
-
 Pseudomonas sp. 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
1.13.11.2 4-chlorocatechol + O2 weak Pseudomonas sp. 5-chloro-2-hydroxymuconic semialdehyde
-
 ?
1.13.11.2 4-methylcatechol + O2
-
 Pseudomonas sp. 2-hydroxy-3-methyl-6-oxohexa-2,4-dienoate
-
 ?
1.13.11.2 catechol + O2
-
 Pseudomonas sp. 2-hydroxymuconate semialdehyde
-
 ?
1.13.11.2 catechol + O2 catechol 2,3-dioxygenase is a key enzyme in the catabolism of monocyclic aromatic compounds including aniline and catalyzes the extradiol cleavage of catechol Pseudomonas sp. 2-hydroxymuconate semialdehyde
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.2 tetramer 4 * 33000 Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.11.2 70
-
 10 min, activity not decreased in the presence of 5% acetone Pseudomonas sp.
1.13.11.2 75
-
 40% loss of original activity Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.2 8 8.5
-
 Pseudomonas sp.