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Literature summary extracted from

  • Kojima, Y.; Itada, N.; Hayaishi, O.
    Metapyrocatechase: a new catechol-cleaving enzyme (1961), J. Biol. Chem., 236, 2223-2228.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.2 Ag+
-
Pseudomonas sp.
1.13.11.2 Hg2+
-
Pseudomonas sp.
1.13.11.2 p-chloromercuribenzoate not Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.2 additional information
-
additional information
-
Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.2 additional information not: Co2+, Mn2+, Cu2+, Cr3+, Zn2+, Ni2+, Cd2+, Ca2+ Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.2 Pseudomonas sp.
-
OC1
-
1.13.11.2 Pseudomonas sp. OC1
-
OC1
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.13.11.2 extremely sensitive to O2, easily inactivated in presence of air Pseudomonas sp.

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.2
-
Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
1.13.11.2 -10°C, 1 week, under nitrogen gas, 72% of the orginal activity Pseudomonas sp.
1.13.11.2 0°C, 4 h, about 30% loss of activity Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.2 catechol + O2 highly specific for Pseudomonas sp. 2-hydroxymuconate semialdehyde
-
?
1.13.11.2 catechol + O2 highly specific for Pseudomonas sp. OC1 2-hydroxymuconate semialdehyde
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.2 7.5
-
-
Pseudomonas sp.