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Literature summary extracted from

  • Iwaki, M.; Nozaki, M.
    Immobilization of metapyrocatechase and its properties in comparison with the soluble enzyme (1982), J. Biochem., 91, 1549-1553.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.13.11.2 immobilization enhances stability against inactivation by heat, acid or alkaline pH and various denaturing agents Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.2 additional information
-
 additional information
-
 Pseudomonas putida
1.13.11.2 0.063
-
 O2 immobilized enzyme Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.2 Pseudomonas putida
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.13.11.2 like native enzyme the immobilized enzyme is rapidly inactivated by oxidants such as O2 or H2O2 Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.2 46
-
 immobilized enzyme Pseudomonas putida
1.13.11.2 270
-
 free enzyme Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.2 3-chlorocatechol + O2
-
 Pseudomonas putida 2-chloro-2-hydroxy-6-oxohexa-2,4-dienoate
-
 ?
1.13.11.2 3-hydroxycatechol + O2
-
 Pseudomonas putida 3-hydroxymuconic acid
-
 ?
1.13.11.2 3-methylcatechol + O2
-
 Pseudomonas putida 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
1.13.11.2 4-methylcatechol + O2 highly specific for Pseudomonas putida 2-hydroxy-3-methyl-6-oxohexa-2,4-dienoate
-
 ?
1.13.11.2 catechol + O2
-
 Pseudomonas putida 2-hydroxymuconate semialdehyde
-
 ?
1.13.11.2 protocatechualdehyde + O2
-
 Pseudomonas putida ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.2 6.5
-
-
 Pseudomonas putida