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Literature summary extracted from

  • Sakakibara, S.; Yamaguchi, K.; Ueda, I.
    Two components of cysteine oxidase in rat liver (1973), Biochem. Biophys. Res. Commun., 52, 1093-1099.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.11.20 hydroxylamine activates, restores the inhibition by Fe2+ Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.20 2,2'-dipyridyl non preincubated protein-B: 76% inhibition at 1 mM, with cysteine preincubated protein-B: no inhibition Rattus norvegicus
1.13.11.20 EDTA non preincubated protein-B: 91% inhibition at 1 mM, with cysteine preincubated protein-B: 51% inhibition at 1 mM Rattus norvegicus
1.13.11.20 Fe2+ inhibits the enzyme activity of both preactivated and non-preactivated protein-B Rattus norvegicus
1.13.11.20 o-phenanthroline non-preincubated protein-B: 91% inhibition at 1 mM, with cysteine preincubated protein-B: 67% inhibition at 1 mM Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.20 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.20 using acid treatment, ammonium sulfate fractionation and column chromatography with DEAE-cellulose. The purified enzyme is composed of two distinct proteins, it appears that one of them is a catalytic protein named protein-B having tightly bound iron as a prosthetic group, while the other is either a modifier or activating protein named protein-A. Protein-B is found to exist in both an active and an inactive form Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.20 liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.20 additional information
-
-
Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.20 L-cysteine + O2
-
Rattus norvegicus 3-sulfino-L-alanine
-
?
1.13.11.20 additional information glutathione, dithiothreitol and cystine do not serve as substrates Rattus norvegicus ?
-
?

Subunits

EC Number Subunits Comment Organism
1.13.11.20 More liver enzyme is composed of 2 distinct proteins: 1. protein-B, tightly bound iron as prosthetic group, 2. protein A, modifier or activating protein Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.20 6.8
-
assay at Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.13.11.20 additional information no requirement of NAD, NADP, NADH or NADPH for the enzymic activity of protein-B. The enzymic activity of protein-B alone is extremely low, protein-A alone does not exhibit catalytic activity, however a significant activity is observed in the presence of both fractions, requirement of protein-A for the catalytic activity of protein-B Rattus norvegicus