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Literature summary extracted from

  • Sharma, H.K.; Vaidyanathan, C.S.
    2,3-Dihydroxybenzoate 2,3-oxygease from the chloroplast fraction of Tecoma stans (1975), Phytochemistry, 14, 2135-2139.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.13.11.28 chloroplast enzyme more stable than soluble enzyme Tecoma stans

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.28 8-hydroxyquinoline reversible by addition of Cu2+ Tecoma stans
1.13.11.28 Ag+
-
 Tecoma stans
1.13.11.28 arsenite
-
 Tecoma stans
1.13.11.28 azide
-
 Tecoma stans
1.13.11.28 Cd2+
-
 Tecoma stans
1.13.11.28 CN-
-
 Tecoma stans
1.13.11.28 Co2+
-
 Tecoma stans
1.13.11.28 diethyldithiocarbamate reversible by addition of Cu2+ Tecoma stans
1.13.11.28 F-
-
 Tecoma stans
1.13.11.28 Fe2+
-
 Tecoma stans
1.13.11.28 Fe3+
-
 Tecoma stans
1.13.11.28 glutathione no inhibition of chloroplast enzyme Tecoma stans
1.13.11.28 Hg2+
-
 Tecoma stans
1.13.11.28 Mg2+ no inhibition of chloroplast enzyme Tecoma stans
1.13.11.28 N-ethylmaleimide
-
 Tecoma stans
1.13.11.28 Neocuproine reversible by addition of Cu2+ Tecoma stans
1.13.11.28 p-chloromercuribenzoate
-
 Tecoma stans
1.13.11.28 Salicylaldoxime reversible by addition of Cu2+ Tecoma stans
1.13.11.28 Semicarbazide
-
 Tecoma stans
1.13.11.28 Sulfide
-
 Tecoma stans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.13.11.28 chloroplast
-
 Tecoma stans 9507
-
1.13.11.28 soluble
-
 Tecoma stans
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.28 2,3-dihydroxybenzoate + O2 Tecoma stans enzyme of the beta-ketoadipate pathway 2-carboxy-cis,cis-muconate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.28 Tecoma stans
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.28 leaf
-
 Tecoma stans
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.28 2,3-dihydroxybenzoate + O2
-
 Tecoma stans 2-carboxy-cis,cis-muconate
-
 ?
1.13.11.28 2,3-dihydroxybenzoate + O2 enzyme of the beta-ketoadipate pathway Tecoma stans 2-carboxy-cis,cis-muconate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.28 30
-
-
 Tecoma stans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.13.11.28 60
-
 30% of activity Tecoma stans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.11.28
-
-
 half-life of chloroplast enzyme is 8-9 h Tecoma stans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.28 5.6
-
-
 Tecoma stans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.13.11.28 7
-
 no activity above pH 7 Tecoma stans