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Literature summary extracted from
- Key aromatic-ring-cleaving enzyme, protocatechuate 3,4-dioxygenase, in the ecologically important marine Roseobacter lineage (2000), Appl. Environ. Microbiol., 66, 4662-4672.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.13.11.3 | expression in Escherichia coli | Sagittula stellata |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.3 | 21900 | - |
x * 21900 + x * 26700, deduced from nucleotide sequences of putative alpha and beta subunits | Sagittula stellata |
| 1.13.11.3 | 26700 | - |
x * 21900 + x * 26700, deduced from nucleotide sequences of putative alpha and beta subunits | Sagittula stellata |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.13.11.3 | Roseobacter sp. | - |
isolates GAI-16, S25com04 and IC4, enzyme activity is induced by growth on p-hydroxybenzoate | - |
| 1.13.11.3 | Sagittula stellata | - |
enzyme activity is induced by growth on p-hydroxybenzoate | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.3 | 0.113 | - |
activity in E. coli cell extracts expressing the recombinant enzyme | Sagittula stellata |
| 1.13.11.3 | 0.31 | - |
Rosebacter isolate GAI-16, enzyme activity in cell extracts | Roseobacter sp. |
| 1.13.11.3 | 0.46 | - |
enzyme activity in cell extracts | Sagittula stellata |
| 1.13.11.3 | 0.73 | - |
Rosebacter isolate S25com04, enzyme activity in cell extracts | Roseobacter sp. |
| 1.13.11.3 | 0.88 | - |
Rosebacter isolate IC4, enzyme activity in cell extracts | Roseobacter sp. |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.13.11.3 | 3,4-dihydroxybenzoate + O2 | - |
Roseobacter sp. | 3-carboxy-cis,cis-muconate | - |
? |  |
| 1.13.11.3 | 3,4-dihydroxybenzoate + O2 | - |
Sagittula stellata | 3-carboxy-cis,cis-muconate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.13.11.3 | ? | x * 21900 + x * 26700, deduced from nucleotide sequences of putative alpha and beta subunits | Sagittula stellata |
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