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Literature summary extracted from

  • Bull, C.; Ballou, D.P.
    Purification and properties of protocatechuate 3,4-dioxygenase from Pseudomonas putida. A new iron to subunit stoichiometry (1981), J. Biol. Chem., 256, 12673-12680.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.3 acetate
-
 Pseudomonas putida
1.13.11.3 Cl-
-
 Pseudomonas putida
1.13.11.3 F-
-
 Pseudomonas putida
1.13.11.3 I-
-
 Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.3 additional information
-
 additional information measurement of Km at different pH values Pseudomonas putida
1.13.11.3 0.002
-
 protocatechuate at pH 8.3, spectrophotometric Pseudomonas putida
1.13.11.3 0.003
-
 protocatechuate at pH 8.3, oxygen electrode Pseudomonas putida
1.13.11.3 0.07
-
 O2 at pH 8.3, spectrophotometric Pseudomonas putida
1.13.11.3 0.09
-
 O2 at pH 8.3, oxygen electrode Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.3 Fe enzyme contains 4 Fe3+ Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.3 23000
-
 alpha,beta, 4 * 23000 + 4 * 26500, SDS-PAGE Pseudomonas putida
1.13.11.3 26500
-
 alpha,beta, 4 * 23000 + 4 * 26500, SDS-PAGE Pseudomonas putida
1.13.11.3 190000
-
 gel filtration Pseudomonas putida
1.13.11.3 202000
-
 meniscus depletion Pseudomonas putida
1.13.11.3 204000
-
 sedimentation equilibrium Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.3 Pseudomonas putida
-
 previously classified as Pseudomonas fluorescens PHK
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.3 3,4-dihydroxybenzoate + O2
-
 Pseudomonas putida 3-carboxy-cis,cis-muconate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.3 octamer alpha,beta, 4 * 23000 + 4 * 26500, SDS-PAGE Pseudomonas putida

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.3 64.8
-
 protocatechuate oxygen electrode at pH 8.3 Pseudomonas putida
1.13.11.3 74.7
-
 protocatechuate spectrophotometric at pH 8.3 Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.3 10
-
-
 Pseudomonas putida