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Literature summary extracted from

  • Wojtas-Wasilewska, M.; Luterek, J.; Rogalski, J.
    Immobilization of protocatechuate 3,4-dioxygenase from Pleurotus ostreatus on activated porous glass beads (1988), Phytochemistry, 27, 2731-2733.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.13.11.3 enzyme immobilized on porous glass beads is stable over wide ranges of pH and temperature Pleurotus ostreatus

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.3 Pleurotus ostreatus
-
 wood-degrading fungus
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.3
-
 Pleurotus ostreatus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.3 0.34
-
 soluble enzyme Pleurotus ostreatus
1.13.11.3 0.39
-
 enzyme immobilized on glass-beads Pleurotus ostreatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.3 3,4-dihydroxybenzoate + O2
-
 Pleurotus ostreatus 3-carboxy-cis,cis-muconate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.3 37
-
 free enzyme, 9% activity at 60°C, no activity at 80°C Pleurotus ostreatus
1.13.11.3 45
-
 immobilized enzyme Pleurotus ostreatus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.13.11.3 45 80 immobilized enzyme: 78% activity at 60°C, 75% activity at 80°C Pleurotus ostreatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.3 7.5
-
 immobilized enzyme Pleurotus ostreatus
1.13.11.3 8
-
 free enzyme Pleurotus ostreatus