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Literature summary extracted from

  • Fujisawa, H.; Hayaishi, O.
    Protocatechuate 3,4-dioxygenase. I. Crystallization and characterization (1968), J. Biol. Chem., 243, 2673-2681.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.13.11.3 addition of 0.1 ml 1 M 2-mercaptoethanol to 10 ml enzyme solution in 50 mM Tris buffer pH 8.5, rhombic crystals appear after 1 week in a refrigerator, crystallization is also achieved by adding 1 mM iodoacetamide instead of mercaptoethanol or by dialyzing the enzyme against cold, destilled water for 2 days Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.3 2,3-Dihydroxybenzoate
-
Pseudomonas aeruginosa
1.13.11.3 3,4-Dihydroxyacetophenone
-
Pseudomonas aeruginosa
1.13.11.3 3,4-dihydroxyphenylacetic acid
-
Pseudomonas aeruginosa
1.13.11.3 Protocatechualdehyde competitive inhibitor Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.3 0.03
-
protocatechuate
-
Pseudomonas aeruginosa
1.13.11.3 0.043
-
O2
-
Pseudomonas aeruginosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.3 Fe enzyme contains 7.3 g-atom of nonheme iron per mol of enzyme Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.3 700000
-
diffusion data Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.3 Pseudomonas aeruginosa
-
nduced in cells grown on 4-hydroxybenzoate
-
1.13.11.3 Pseudomonas aeruginosa B-10
-
nduced in cells grown on 4-hydroxybenzoate
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.3
-
Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.3 61.6
-
-
Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.3 3,4-dihydroxybenzoate + O2 very low activity with: 3,4-dihydroxyphenylacetic acid Pseudomonas aeruginosa 3-carboxy-cis,cis-muconate
-
?
1.13.11.3 3,4-dihydroxybenzoate + O2 very low activity with 3,4-dihydroxymandelic acid Pseudomonas aeruginosa 3-carboxy-cis,cis-muconate
-
?
1.13.11.3 3,4-dihydroxybenzoate + O2 very low activity with 4'-methylcatechol Pseudomonas aeruginosa 3-carboxy-cis,cis-muconate
-
?
1.13.11.3 3,4-dihydroxybenzoate + O2 very low activity with: 3,4-dihydroxyphenylacetic acid Pseudomonas aeruginosa B-10 3-carboxy-cis,cis-muconate
-
?
1.13.11.3 3,4-dihydroxybenzoate + O2 very low activity with 3,4-dihydroxymandelic acid Pseudomonas aeruginosa B-10 3-carboxy-cis,cis-muconate
-
?
1.13.11.3 3,4-dihydroxybenzoate + O2 very low activity with 4'-methylcatechol Pseudomonas aeruginosa B-10 3-carboxy-cis,cis-muconate
-
?
1.13.11.3 3-methylcatechol + O2 at 0.4% the rate of protocatechuic acid oxidation Pseudomonas aeruginosa 2-methylmuconate
-
?
1.13.11.3 catechol + O2 at 0.4% the rate of protocatechuic acid oxidation Pseudomonas aeruginosa muconate
-
?
1.13.11.3 catechol + O2 at 0.4% the rate of protocatechuic acid oxidation Pseudomonas aeruginosa B-10 muconate
-
?
1.13.11.3 pyrogallol + O2 at 0.4% the rate of protocatechuic acid oxidation Pseudomonas aeruginosa ?
-
?
1.13.11.3 pyrogallol + O2 at 0.4% the rate of protocatechuic acid oxidation Pseudomonas aeruginosa B-10 ?
-
?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.11.3 60
-
5 min, about 5% loss of activity Pseudomonas aeruginosa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.3 758
-
3,4-dihydroxybenzoate
-
Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.3 8
-
-
Pseudomonas aeruginosa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.13.11.3 6 9.6 24°C, 1 week, less than 10% loss of activity Pseudomonas aeruginosa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.13.11.3 0.014
-
Protocatechualdehyde
-
Pseudomonas aeruginosa