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Literature summary extracted from

  • Murakami, S.; Okuno, T.; Matsumura, E.; Takenaka, S.; Shinke, R.; Aoki, K.
    Cloning of a gene encoding hydroxyquinol 1,2-dioxygenase that catalyzes both intradiol and extradiol ring cleavage of catechol (1999), Biosci. Biotechnol. Biochem., 63, 859-865.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.37 expressed in Escherichia coli Arthrobacter sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.37 AgNO3
-
 Arthrobacter sp.
1.13.11.37 HgCl2
-
 Arthrobacter sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.37 36000
-
 ? * 36000, SDS-PAGE Arthrobacter sp.
1.13.11.37 79000
-
 gel filtration Arthrobacter sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.37 benzene-1,2,4-triol + O2 Arthrobacter sp.
-
 3-hydroxy-cis-cis-muconate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.37 Arthrobacter sp. Q9S1B4 BA-5-17, grown in benzamide medium
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.37
-
 Arthrobacter sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.37 additional information
-
-
 Arthrobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.37 benzene-1,2,4-triol + O2 6.8-fold activity of that for catechol Arthrobacter sp. maleylacetate
-
 ?
1.13.11.37 benzene-1,2,4-triol + O2
-
 Arthrobacter sp. 3-hydroxy-cis-cis-muconate
-
 ?
1.13.11.37 catechol + O2 intradiol ring cleavage Arthrobacter sp. cis,cis-muconic acid
-
 ?
1.13.11.37 catechol + O2 extradiol ring cleavage Arthrobacter sp. 2-hydroxymuconic 6-semialdehyde
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.37 ? ? * 36000, SDS-PAGE Arthrobacter sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.11.37 50
-
 activity not influenced after incubation for 10 min Arthrobacter sp.
1.13.11.37 55
-
 60% activity loss after 10 min Arthrobacter sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.37 8.5
-
 for catechol Arthrobacter sp.