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Literature summary extracted from

  • Imbeault, N.Y.; Powlowski, J.B.; Colbert, C.L.; Bolin, J.T.; Eltis, L.D.
    Steady-state kinetic characterization and crystallization of a polychlorinated biphenyl-transforming dioxygenase (2000), J. Biol. Chem., 275, 12430-12437.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.39
-
 Comamonas testosteroni
1.14.12.18 in Pseudomonas putida strain KT2442 Comamonas testosteroni

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.13.11.39 space group R3, a = b = 136.35 A, c = 106.07 A Comamonas testosteroni

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.39 0.0026
-
 2,2'-dichlorobiphenyl
-
 Comamonas testosteroni
1.13.11.39 0.0033
-
 3,3'-dichlorobiphenyl
-
 Comamonas testosteroni
1.13.11.39 0.0062
-
 biphenyl
-
 Comamonas testosteroni
1.13.11.39 0.028
-
 O2 biphenyl as substrate Comamonas testosteroni
1.13.11.39 0.19
-
 O2 3,3'-dichlorobiphenyl as substrate Comamonas testosteroni
1.13.11.39 0.75
-
 O2 2,2'-dichlorobiphenyl as substrate Comamonas testosteroni
1.14.12.18 0.0026
-
 2,2'-dichlorobiphenyl recombinant, purified protein Comamonas testosteroni
1.14.12.18 0.0033
-
 3,3'-dichlorobiphenyl recombinant, purified protein Comamonas testosteroni
1.14.12.18 0.0062
-
 biphenyl recombinant, purified protein Comamonas testosteroni
1.14.12.18 0.028
-
 O2 recombinant, purified protein Comamonas testosteroni

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.39 Fe2+
-
 Pseudomonas putida
1.13.11.39 Fe2+
-
 Comamonas testosteroni

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.39 biphenyl-2,3-diol + O2 Pseudomonas putida
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
1.13.11.39 biphenyl-2,3-diol + O2 Comamonas testosteroni
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
1.13.11.39 biphenyl-2,3-diol + O2 Pseudomonas oleovorans
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
1.13.11.39 biphenyl-2,3-diol + O2 Pseudomonas putida KT 2442
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
1.13.11.39 biphenyl-2,3-diol + O2 Pseudomonas oleovorans KF707
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.39 Comamonas testosteroni
-
-
-
1.13.11.39 Pseudomonas oleovorans
-
-
-
1.13.11.39 Pseudomonas oleovorans KF707
-
-
-
1.13.11.39 Pseudomonas putida
-
-
-
1.13.11.39 Pseudomonas putida KT 2442
-
-
-
1.14.12.18 Comamonas testosteroni
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.39
-
 Comamonas testosteroni
1.14.12.18 purification from heterologous expression in Pseudomonas putida KT2442 Comamonas testosteroni

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.39 4.9
-
-
 Comamonas testosteroni
1.14.12.18 4.9
-
 recombinant protein after purification Comamonas testosteroni

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.39 2,2'-dichlorobiphenyl + O2
-
 Comamonas testosteroni ?
-
 ?
1.13.11.39 3,3'-dichlorobiphenyl + O2
-
 Comamonas testosteroni ?
-
 ?
1.13.11.39 4,4'-dichlorobiphenyl + O2
-
 Comamonas testosteroni ?
-
 ?
1.13.11.39 biphenyl + O2
-
 Comamonas testosteroni ?
-
 ?
1.13.11.39 biphenyl-2,3-diol + O2
-
 Pseudomonas putida 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
1.13.11.39 biphenyl-2,3-diol + O2
-
 Comamonas testosteroni 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
1.13.11.39 biphenyl-2,3-diol + O2
-
 Pseudomonas oleovorans 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
1.13.11.39 biphenyl-2,3-diol + O2
-
 Pseudomonas putida KT 2442 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
1.13.11.39 biphenyl-2,3-diol + O2
-
 Pseudomonas oleovorans KF707 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.39 BPDO
-
 Comamonas testosteroni