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Literature summary extracted from

  • Decker, R.H.; Kang, H.H.; Leach, F.R.; Henderson, L.M.
    Purification and properties of 3-hydroxyanthranilic acid oxidase (1961), J. Biol. Chem., 236, 3076-3082.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.11.6 Triton X-100
-
Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.6 p-chloromercuribenzoate
-
Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.6 0.021
-
3-hydroxyanthranilic acid
-
Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.6 (NH4)2SO4 the most effective salt, the optimal concentration is 0.035 M Bos taurus
1.13.11.6 Fe2+ solvent, acid and heat function to modify the protein configuration so that ferrous ions can be bound to the enzyme to generate the most active form Bos taurus
1.13.11.6 HCl during purification of enzyme treatment with acid was used Bos taurus
1.13.11.6 MgCl2
-
Bos taurus
1.13.11.6 NaCl
-
Bos taurus

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.13.11.6 additional information drastic change in the Km of the enzyme in the presence of dimethylglutarate buffer Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.6 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.6
-
Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.6 liver
-
Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.6 additional information
-
reaches a maximum after 10 min at acid pH Bos taurus

Storage Stability

EC Number Storage Stability Organism
1.13.11.6 -15°C, in 0.01 M Tris buffer, pH 7.0, 4 days, no loss of activity Bos taurus
1.13.11.6 4°C, overnight, about 75% loss of activity Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.6 3-hydroxyanthranilate + O2
-
Bos taurus 2-amino-3-carboxymuconate semialdehyde
-
?

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.13.11.6 55
-
activation by heating at 55°C for 5 min Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.13.11.6 additional information
-
at pH 3.4: the enzyme is in a form which can bind substrate but is enzymatically inactive, at pH 6.5: active form of enzyme Bos taurus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.13.11.6 10
-
4°C, half-life: 3 days Bos taurus