Any feedback?
Literature summary extracted from
- Kinetic and Mossbauer studies on the mechanism of protocatechuic acid 4,5-oxygenase (1972), Biochemistry, 11, 3212-3219.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.13.11.8 | 4-nitrocatechol | competitive inhibitor | Comamonas testosteroni |  |
| 1.13.11.8 | iodoacetamide | competitive inhibitor | Comamonas testosteroni | |
| 1.13.11.8 | p-chloromercuribenzoic acid | excellent competitive inhibitor | Comamonas testosteroni | |
| 1.13.11.8 | Protocatechualdehyde | competitive inhibitor | Comamonas testosteroni | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.13.11.8 | 0.046 | - |
protocatechuate | - |
Comamonas testosteroni | |
| 1.13.11.8 | 0.0735 | - |
gallic acid | - |
Comamonas testosteroni | |
| 1.13.11.8 | 0.125 | - |
5-methoxygallic acid | - |
Comamonas testosteroni | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.13.11.8 | Fe2+ | four iron atoms per molecule | Comamonas testosteroni | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.8 | 140000 | - |
- |
Comamonas testosteroni |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.13.11.8 | protocatechuate + O2 | Comamonas testosteroni | - |
4-carboxy-2-hydroxymuconate semialdehyde | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.13.11.8 | Comamonas testosteroni | - |
grown on p-hydroxybenzoate-containing medium | - |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 1.13.11.8 | 3,4-dihydroxybenzoate + O2 = 4-carboxy-2-hydroxymuconate semialdehyde | involvement of sulfhydryl groups at the active site, 2 free sulfhydryl groups per enzyme molecule | Comamonas testosteroni |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.13.11.8 | 5-methoxygallic acid + O2 | - |
Comamonas testosteroni | ? | - |
? | |
| 1.13.11.8 | gallic acid + O2 | - |
Comamonas testosteroni | ? | - |
? | |
| 1.13.11.8 | protocatechuate + O2 | - |
Comamonas testosteroni | 4-carboxy-2-hydroxymuconate semialdehyde | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.8 | 30 | - |
enzyme assay | Comamonas testosteroni |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.13.11.8 | 0.56 | - |
4-nitrocatechol | - |
Comamonas testosteroni | |
| 1.13.11.8 | 0.96 | - |
Protocatechualdehyde | - |
Comamonas testosteroni | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
