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Literature summary extracted from

  • Arciero, D.M.; Orville, A.M.; Lipscomb, J.D.
    Protocatechuate 4,5-dioxygenase from Pseudomonas testosteroni (1990), Methods Enzymol., 188, 89-95.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.13.11.8
-
 Comamonas testosteroni

General Stability

EC Number General Stability Organism
1.13.11.8 dialysis can be performed only at temperatures at or below 4°C Comamonas testosteroni

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.8 0.08
-
 protocatechuate
-
 Comamonas testosteroni
1.13.11.8 0.125
-
 O2
-
 Comamonas testosteroni

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.8 Fe2+ present at a significant mol fraction, metal-free enzyme is inactive, iron can be removed by treatment with tiron Comamonas testosteroni
1.13.11.8 Fe2+ iron is present in active enzyme in the high-spin ferrous state Comamonas testosteroni

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.8 17700
-
 alpha2, beta2. 2 * 17700 + 2 * 33800, dimer of a heterodimer, SDS-PAGE Comamonas testosteroni
1.13.11.8 33800
-
 alpha2, beta2. 2 * 17700 + 2 * 33800, dimer of a heterodimer, SDS-PAGE Comamonas testosteroni
1.13.11.8 142000
-
 gel filtration Comamonas testosteroni

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.8 protocatechuate + O2 Comamonas testosteroni
-
 4-carboxy-2-hydroxymuconate semialdehyde
-
 ?
1.13.11.8 protocatechuate + O2 Comamonas testosteroni Pt-L5 / ATCC 49249
-
 4-carboxy-2-hydroxymuconate semialdehyde
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.8 Comamonas testosteroni
-
-
-
1.13.11.8 Comamonas testosteroni
-
 grown on p-hydroxybenzoate-containing medium
-
1.13.11.8 Comamonas testosteroni Pt-L5 / ATCC 49249
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.13.11.8 rapidly inactivated by oxidizing agents such as H2O2 or ferricyanide, partial reactivation by ascorbate Comamonas testosteroni

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.8
-
 Comamonas testosteroni

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.8 212
-
-
 Comamonas testosteroni

Storage Stability

EC Number Storage Stability Organism
1.13.11.8 -196°C, in liquid nitrogen, without significant loss of activity for several years Comamonas testosteroni

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.8 5-methylprotocatechuate + O2
-
 Comamonas testosteroni 4-carboxy-2-hydroxy-3-methylmuconate semialdehyde
-
 ?
1.13.11.8 5-methylprotocatechuate + O2
-
 Comamonas testosteroni Pt-L5 / ATCC 49249 4-carboxy-2-hydroxy-3-methylmuconate semialdehyde
-
 ?
1.13.11.8 protocatechuate + O2
-
 Comamonas testosteroni 4-carboxy-2-hydroxymuconate semialdehyde
-
 ?
1.13.11.8 protocatechuate + O2
-
 Comamonas testosteroni Pt-L5 / ATCC 49249 4-carboxy-2-hydroxymuconate semialdehyde
-
 ?
1.13.11.8 sulfonylcatechol + O2
-
 Comamonas testosteroni ?
-
 ?
1.13.11.8 sulfonylcatechol + O2
-
 Comamonas testosteroni Pt-L5 / ATCC 49249 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.8 tetramer alpha2, beta2. 2 * 17700 + 2 * 33800, dimer of a heterodimer, SDS-PAGE Comamonas testosteroni

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.8 350
-
 protocatechuate
-
 Comamonas testosteroni

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.8 8 8.5
-
 Comamonas testosteroni