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Literature summary extracted from

  • Koyama, H.
    Further characterization of a novel L-phenylalanine oxidase (deaminating and decarboxylating) from Pseudomonas sp. P-501 (1983), J. Biochem., 93, 1313-1319.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.12.9 1,10-phenanthroline slight Pseudomonas sp.
1.13.12.9 2,2'-dipyridyl slight Pseudomonas sp.
1.13.12.9 8-hydroxyquinoline slight Pseudomonas sp.
1.13.12.9 CuSO4 preferentially inhibits oxidase activity versus oxygenase activity Pseudomonas sp.
1.13.12.9 FeSO4 preferentially inhibits oxidase activity versus oxygenase activity Pseudomonas sp.
1.13.12.9 HgCl2
-
 Pseudomonas sp.
1.13.12.9 ZnSO4
-
 Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.12.9 0.0107
-
 L-Phe
-
 Pseudomonas sp.
1.13.12.9 1.82
-
 O2
-
 Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.12.9 68000
-
 2 * 68000, SDS-PAGE Pseudomonas sp.
1.13.12.9 140000
-
 gel filtration Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
1.13.12.9 Pseudomonas sp.
-
 P-501
-
1.13.12.9 Pseudomonas sp. P-501
-
 P-501
-

Storage Stability

EC Number Storage Stability Organism
1.13.12.9 -20°C, 1 mM or 10 mM potassium phosphate buffer, pH 7.0, stable for at least 6 months Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.12.9 L-Phe + O2
-
 Pseudomonas sp. 2-phenylacetamide + CO2 + H2O
-
 ?
1.13.12.9 L-Phe + O2
-
 Pseudomonas sp. P-501 2-phenylacetamide + CO2 + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.12.9 dimer 2 * 68000, SDS-PAGE Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.12.9 45
-
 formation of alpha-phenylacetamide Pseudomonas sp.
1.13.12.9 65
-
 formation of beta-phenylpyruvate Pseudomonas sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.13.12.9 20 70 20°C: about 65% of maximal activity, 70°C: about 50% of maximal activity, formation of alpha-phenylacetamide Pseudomonas sp.
1.13.12.9 40 85 40°C: about 50% of maximal activity 40°C and 85°C, formation of beta-phenylpyruvate Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.12.9 60
-
 10 min, no loss of activity Pseudomonas sp.
1.13.12.9 80
-
 10 min, 46% loss of activity Pseudomonas sp.
1.13.12.9 85
-
 10 min, 90% loss of activity Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.12.9 6 9.5 formation of alpha-phenylacetamide Pseudomonas sp.
1.13.12.9 11
-
 formation of beta-phenylpyruvate Pseudomonas sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.13.12.9 9.8 11.8 50% of maximal activity at pH 9.8 and 11.8, formation of beta-phenylpyruvate Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.13.12.9 FAD contains 2 mol of FAD per mol of enzyme Pseudomonas sp.