EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.13.99.3 | hydroxylamine | - |
Pseudomonas fluorescens | |
1.13.99.3 | indole-3-methanol | inhibits oxidation of skatole | Pseudomonas fluorescens | |
1.13.99.3 | KCN | - |
Pseudomonas fluorescens | |
1.13.99.3 | skatole | inhibits oxidation of indole-3-methanol | Pseudomonas fluorescens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.13.99.3 | 0.48 | - |
indole-3-methanol | - |
Pseudomonas fluorescens | |
1.13.99.3 | 0.67 | - |
ferricyanide | - |
Pseudomonas fluorescens |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.13.99.3 | Fe | dehydrogenase component: 0.67 atoms of iron per mol of component | Pseudomonas fluorescens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.13.99.3 | 48000 | - |
1 * 72000 (dehydrogenase component) + 1 * 48000 (oxidase component), TSO II, SDS-PAGE | Pseudomonas fluorescens |
1.13.99.3 | 72000 | - |
1 * 72000 (dehydrogenase component) + 1 * 48000 (oxidase component), TSO II, SDS-PAGE | Pseudomonas fluorescens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.13.99.3 | Pseudomonas fluorescens | - |
ATCC 29574 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.13.99.3 | dehydrogenase component and oxidase component | Pseudomonas fluorescens |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.13.99.3 | 29.2 | - |
- |
Pseudomonas fluorescens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.99.3 | 3-indolemethanol + ferricyanide | - |
Pseudomonas fluorescens | ? | - |
r | |
1.13.99.3 | 3-indolemethanol + O2 | - |
Pseudomonas fluorescens | ? | - |
? | |
1.13.99.3 | additional information | dehydrogenase component abstracts electrons from the substrate and transfers them to oxidation-reduction dyes (e.g. potassium ferricyanide, 2,6-dichlorophenolindophenol) but not to molecular oxygen, the oxidase component transfers electrons from the former component to oxygen | Pseudomonas fluorescens | additional information | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.13.99.3 | dimer | 1 * 72000 (dehydrogenase component) + 1 * 48000 (oxidase component), TSO II, SDS-PAGE | Pseudomonas fluorescens |
1.13.99.3 | dimer | dehydrogenase component abstracts electrons from the substrate and transfers them to oxidation-reduction dyes e.g. potassium ferricyanide, 2,6-dichlorophenolindophenol, but not to molecular oxygen, the oxidase component transfers electrons from the former component to oxygen | Pseudomonas fluorescens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.13.99.3 | 25 | - |
assay at | Pseudomonas fluorescens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.13.99.3 | 3.5 | - |
- |
Pseudomonas fluorescens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.13.99.3 | heme | hemoprotein | Pseudomonas fluorescens | |
1.13.99.3 | heme | dehydrogenase component: 0.73 mol protoheme IX per mol of component | Pseudomonas fluorescens |