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Literature summary extracted from

  • Ito, S.; Takai, K.; Tokuyama, T.; Hayaishi, O.
    Enzymatic modification of tryptophan residues by tryptophan side chain oxidase I and II from Pseudomonas (1981), J. Biol. Chem., 256, 7834-7843.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.3.10 150000
-
-
 Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
1.3.3.10 Pseudomonas sp.
-
-
-
1.13.99.3 Pseudomonas fluorescens
-
 ATCC 29574
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.3.10 gel filtration, phosphocellulose column chromatography, SDS-PAGE Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.3.10 0.02
-
 substrate tryptophanamide Pseudomonas sp.
1.3.3.10 0.07
-
 substrate tryptophan-leucine Pseudomonas sp.
1.3.3.10 0.31
-
 substrate tryptophan Pseudomonas sp.
1.3.3.10 0.74
-
 substrate leucin-tryptophan-leucin Pseudomonas sp.
1.3.3.10 1.08
-
 substrate indolepropionic acid Pseudomonas sp.
1.3.3.10 1.42
-
 substrate N-acetyltryptophanamide Pseudomonas sp.
1.3.3.10 1.82
-
 substrate N-acetyltryptophan Pseudomonas sp.
1.3.3.10 2.32
-
 substrate leucin-tryptophan Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.99.3 L-tryptophan + O2
-
 Pseudomonas fluorescens 3-indoleglycolaldehyde + CO2 + NH3
-
 ?
1.13.99.3 Leu-Trp + O2
-
 Pseudomonas fluorescens Leu-alpha,beta-dehydrotryptamine + CO2 + H2O
-
 ?
1.13.99.3 Leu-Trp-Leu + O2
-
 Pseudomonas fluorescens Leu-beta-hydroxy-Trp-Leu + Leu-beta-hydroxy-Trp-Leu + Leu-alpha,beta-dehydro-Trp-Leu + ? ratio of products depends on pH and ionic strength ?
1.13.99.3 additional information substrate: yeast mating hormone (alpha-factor) Pseudomonas fluorescens additional information with any substrate 3-indolecarboxaldehyde is a sole detectable by-product ?
1.13.99.3 Trp-Leu + O2
-
 Pseudomonas fluorescens threo-beta-hydroxytryptophan + erythro-beta-hydroxytryptophan + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.3.10 tryptophan side chain oxidase II
-
 Pseudomonas sp.