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Literature summary extracted from

  • Takai, K.; Ushiro, H.; Noda, Y.; Narumiya, S.; Tokuyama, T.; Hayaishi, O.
    cristalline hemoprotein from Pseudomonas that catalyzes oxidation of side chain of tryptophan and other indole derivatives (1977), J. Biol. Chem., 252, 2638-2656.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.99.3 additional information enzyme is activated 1.5fold and 3fold in presence of 8 M urea and 0.5% SDS respectively Pseudomonas sp.

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.13.99.3
-
 Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.99.3 CO weak Pseudomonas sp.
1.13.99.3 hydroxylamine
-
 Pseudomonas sp.
1.13.99.3 KCN
-
 Pseudomonas sp.
1.13.99.3 Sodium azide weak Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.99.3 0.0015
-
 L-tryptophan at pH 6.0 Pseudomonas sp.
1.13.99.3 0.004
-
 L-tryptophan at pH 3.0 Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.99.3 280000
-
 gel filtration Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
1.13.99.3 Pseudomonas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.99.3
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.99.3 1.3
-
-
 Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
1.13.99.3 -80°C, several months Pseudomonas sp.
1.13.99.3 0°C, pH 6, stable in presence of either 80% ethyl alcohol, 6 M urea or 0.5% SDS for at least 6 h Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.99.3 3-indoleacetamide + O2
-
 Pseudomonas sp. ?
-
 ?
1.13.99.3 3-indolelactate + O2
-
 Pseudomonas sp. ?
-
 ?
1.13.99.3 3-indolepropionate + O2
-
 Pseudomonas sp. ?
-
 ?
1.13.99.3 3-indolethanol + O2
-
 Pseudomonas sp. ?
-
 ?
1.13.99.3 3-methylindole + O2
-
 Pseudomonas sp. 3-indolecarboxaldehyde + H2O
-
 ?
1.13.99.3 D-tryptophan + O2
-
 Pseudomonas sp. ?
-
 ?
1.13.99.3 L-tryptophan + O2
-
 Pseudomonas sp. 3-indoleglycolaldehyde + CO2 + NH3
-
 ?
1.13.99.3 melatonin + O2
-
 Pseudomonas sp. ?
-
 ?
1.13.99.3 additional information overview: specificity Pseudomonas sp. additional information
-
 ?
1.13.99.3 N-acetyl-L-tryptophan + O2
-
 Pseudomonas sp. ?
-
 ?
1.13.99.3 N-acetyl-L-tryptophanamide + O2
-
 Pseudomonas sp. N-acetyl-alpha,beta-didehydrotryptophanamide + H2O
-
 ?
1.13.99.3 N-acetylacetamide + O2
-
 Pseudomonas sp. ?
-
 ?
1.13.99.3 tryptamine + O2
-
 Pseudomonas sp. ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.99.3 25
-
 assay at Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.99.3 80
-
-
 Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.99.3 3
-
-
 Pseudomonas sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.13.99.3 2
-
-
 Pseudomonas sp.
1.13.99.3 6
-
-
 Pseudomonas sp.
1.13.99.3 11
-
-
 Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.13.99.3 heme hemoprotein Pseudomonas sp.