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Literature summary extracted from

  • Tanaka, M.; Shibata, H.; Uchida, T.
    A new prolyl hydroxylase acting on poly-L-proline, from suspension cultured cells of Vinca rosea (1980), Biochim. Biophys. Acta, 616, 188-198.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.11.2 bovine serum albumin activation Catharanthus roseus
1.14.11.2 dithiothreitol activation Catharanthus roseus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.11.2 additional information high concentrations of salts are inhibitory Catharanthus roseus
1.14.11.2 NaCl more than 0.3 M Catharanthus roseus
1.14.11.2 poly-L-hydroxyproline MW: 30000, inhibitory Catharanthus roseus
1.14.11.2 potassium phosphate
-
 Catharanthus roseus
1.14.11.2 Sodium acetate
-
 Catharanthus roseus
1.14.11.2 succinate 11.5% inhibition at 0.5 mM and 38.3% inhibition at 3 mM Catharanthus roseus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.11.2 0.06
-
 O2
-
 Catharanthus roseus
1.14.11.2 0.23
-
 poly(L-proline)
-
 Catharanthus roseus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.11.2 microsome 40.7% of total activity Catharanthus roseus
-
-
1.14.11.2 mitochondrion 47.9% of total activity Catharanthus roseus 5739
-
1.14.11.2 soluble 11.4% of total activity Catharanthus roseus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.2 Fe2+
-
 Catharanthus roseus

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.2 Catharanthus roseus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.11.2 using ammonium sulfate precipitation and column chromatography on DEAE-Sephadex A-50 Catharanthus roseus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.11.2 cell suspension culture
-
 Catharanthus roseus
-

Storage Stability

EC Number Storage Stability Organism
1.14.11.2 -80°C, dialyzed against 25% glycerol, 1 month Catharanthus roseus
1.14.11.2 4°C, unstable Catharanthus roseus
1.14.11.2 freeze-thawing, unstable Catharanthus roseus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.2 (Pro-Pro-Gly)n + 2-oxoglutarate + O2 n: 5,10, poor substrates Catharanthus roseus (Pro-4-hydroxy-Pro-Gly)n + succinate + CO2
-
 ?
1.14.11.2 Gly-Pro8 + 2-oxoglutarate + O2 96% relative activity with respect to poly(L-proline) of MW 2000 Catharanthus roseus 4-hydroxyproline containing peptide
-
 ?
1.14.11.2 additional information no reaction with: free proline, prolyl peptides whose residues number is four or less Catharanthus roseus ?
-
 ?
1.14.11.2 octa-L-proline + 2-oxoglutarate + O2 81.8% relative activity with respect to poly(L-proline) of MW 2000 Catharanthus roseus 4-hydroxyproline containing peptide
-
 ?
1.14.11.2 poly(L-Pro) + 2-oxoglutarate + O2 MW 2000, 6000 and 12000 Catharanthus roseus poly(4-hydroxyproline) + succinate + CO2
-
 ?
1.14.11.2 poly(L-proline) + 2-oxoglutarate + O2
-
 Catharanthus roseus poly(trans-4-hydroxy-L-proline) + succinate + CO2
-
 r
1.14.11.2 tert-butyloxycarbonyl-Pro8 + 2-oxoglutarate + O2 91.4% relative activity with respect to poly(L-proline) of MW 2000 Catharanthus roseus 4-hydroxyproline containing peptide
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.11.2 6.8
-
-
 Catharanthus roseus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.11.2 2-oxoglutarate Km: 0.09 mM Catharanthus roseus
1.14.11.2 ascorbate
-
 Catharanthus roseus
1.14.11.2 additional information alpha-ketoglutarate cannot be replaced by pyruvate and oxaloacetate Catharanthus roseus