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Literature summary extracted from

  • Chrispeels, M.J.
    Prolyl hydroxylase in plants (1984), Methods Enzymol., 107, 361-369.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.11.2 bovine serum albumin activation Catharanthus roseus
1.14.11.2 dithiothreitol activation Catharanthus roseus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.11.2 potassium phosphate
-
 Catharanthus roseus
1.14.11.2 Sodium acetate
-
 Catharanthus roseus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.11.2 0.06
-
 O2
-
 Catharanthus roseus
1.14.11.2 0.23
-
 poly(L-proline)
-
 Catharanthus roseus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.11.2 endoplasmic reticulum 30% of the enzyme activity Lolium multiflorum 5783
-
1.14.11.2 Golgi apparatus 70% of the enzyme activity Lolium multiflorum 5794
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.2 Fe2+
-
 Daucus carota
1.14.11.2 Fe2+
-
 Catharanthus roseus
1.14.11.2 Fe2+
-
 Lolium multiflorum

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.2 Catharanthus roseus
-
-
-
1.14.11.2 Daucus carota
-
-
-
1.14.11.2 Lolium multiflorum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.11.2 partial, using ammonium sulfate precipitation, column chromatography on DEAE-Sephadex A-50 and affinity chromatography on agarose-linked poly(L-proline) column Catharanthus roseus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.2 (Pro-Pro-Gly)n + 2-oxoglutarate + O2
-
 Catharanthus roseus (Pro-4-hydroxy-Pro-Gly)n + succinate + CO2
-
 ?
1.14.11.2 Gly-Pro8 + 2-oxoglutarate + O2 96% relative activity with respect to poly(L-proline) of MW 2000 Catharanthus roseus 4-hydroxyproline containing peptide
-
 ?
1.14.11.2 additional information no reaction with: free proline, prolyl peptides whose residues number is four or less Daucus carota ?
-
 ?
1.14.11.2 additional information no reaction with: free proline, prolyl peptides whose residues number is four or less Catharanthus roseus ?
-
 ?
1.14.11.2 additional information no reaction with: free proline, prolyl peptides whose residues number is four or less Lolium multiflorum ?
-
 ?
1.14.11.2 octa-L-proline + 2-oxoglutarate + O2 81.8% relative activity with respect to poly(L-proline) of MW 2000 Catharanthus roseus 4-hydroxyproline containing peptide
-
 ?
1.14.11.2 poly(L-Pro) + 2-oxoglutarate + O2
-
 Lolium multiflorum poly(4-hydroxyproline) + succinate + CO2
-
 ?
1.14.11.2 poly(L-Pro) + 2-oxoglutarate + O2 MW 2000, 6000 and 12000 Catharanthus roseus poly(4-hydroxyproline) + succinate + CO2
-
 ?
1.14.11.2 poly(L-proline) + 2-oxoglutarate + O2
-
 Catharanthus roseus poly(trans-4-hydroxy-L-proline) + succinate + CO2
-
 r
1.14.11.2 protocollagen + 2-oxoglutarate + O2
-
 Daucus carota 4-hydroxyproline containing protocollagen + succinate + CO2
-
 ?
1.14.11.2 protocollagen + 2-oxoglutarate + O2
-
 Catharanthus roseus 4-hydroxyproline containing protocollagen + succinate + CO2
-
 ?
1.14.11.2 protocollagen + 2-oxoglutarate + O2
-
 Lolium multiflorum 4-hydroxyproline containing protocollagen + succinate + CO2
-
 ?
1.14.11.2 tert-butyloxycarbonyl-Pro8 + 2-oxoglutarate + O2 91.4% relative activity with respect to poly(L-proline) of MW 2000 Catharanthus roseus 4-hydroxyproline containing peptide
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.11.2 dimer
-
 Catharanthus roseus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.11.2 6.8
-
-
 Catharanthus roseus
1.14.11.2 7.5
-
-
 Lolium multiflorum

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.11.2 2-oxoglutarate Km: 0.09 mM Catharanthus roseus
1.14.11.2 ascorbate
-
 Daucus carota
1.14.11.2 ascorbate
-
 Catharanthus roseus
1.14.11.2 ascorbate
-
 Lolium multiflorum