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Literature summary extracted from

  • Kaska, D.D.; Gunzler, V.; Kivirikko, K.I.; Myllylä, R.
    Characterization of a low-relative-molecular-mass prolyl 4-hydroxylase from the green alga Chlamydomonas reinhardii (1987), Biochem. J., 241, 483-490.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.11.2 bovine serum albumin activation Chlamydomonas reinhardtii
1.14.11.2 catalase activation Chlamydomonas reinhardtii
1.14.11.2 D-isoascorbate can partially replace ascorbate Chlamydomonas reinhardtii
1.14.11.2 dithiothreitol activation Chlamydomonas reinhardtii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.11.2 2-oxoadipate
-
 Chlamydomonas reinhardtii
1.14.11.2 2-oxosuccinate i.e. oxaloacetate Chlamydomonas reinhardtii
1.14.11.2 3,4-dihydroxybenzoate
-
 Chlamydomonas reinhardtii
1.14.11.2 Benzene 1,3-dicarboxylate
-
 Chlamydomonas reinhardtii
1.14.11.2 Glutarate
-
 Chlamydomonas reinhardtii
1.14.11.2 Pyridine 2,4-dicarboxylate
-
 Chlamydomonas reinhardtii
1.14.11.2 Pyridine 2,5-dicarboxylate
-
 Chlamydomonas reinhardtii
1.14.11.2 pyridine 2-carboxylate
-
 Chlamydomonas reinhardtii
1.14.11.2 pyruvate
-
 Chlamydomonas reinhardtii
1.14.11.2 succinate
-
 Chlamydomonas reinhardtii
1.14.11.2 Zn2+
-
 Chlamydomonas reinhardtii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.11.2 0.007
-
 poly(L-Pro) MW 31000 Chlamydomonas reinhardtii
1.14.11.2 0.011
-
 poly(L-Pro) MW 19000 Chlamydomonas reinhardtii
1.14.11.2 0.023
-
 poly(L-Pro) MW 7000 Chlamydomonas reinhardtii
1.14.11.2 0.03
-
 2-oxoglutarate
-
 Chlamydomonas reinhardtii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.2 Fe2+ Km: 0.01-0.03 mM Chlamydomonas reinhardtii
1.14.11.2 Fe2+ Km: 0.028 mM Chlamydomonas reinhardtii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.11.2 40000
-
 gel filtration Chlamydomonas reinhardtii

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.2 Chlamydomonas reinhardtii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.11.2 partial, using ion-exchange chromatography on DEAE-cellulose and gel filtration Chlamydomonas reinhardtii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.2 (Pro-Pro-Gly)n + 2-oxoglutarate + O2 a low hydroxylation rate is found with denatured (Pro-Pro-Gly)10 Chlamydomonas reinhardtii (Pro-4-hydroxy-Pro-Gly)n + succinate + CO2
-
 ?
1.14.11.2 2-oxoglutarate + O2 + ascorbate
-
 Chlamydomonas reinhardtii succinate + dehydroascorbate + CO2 + H2O
-
 ?
1.14.11.2 additional information protocollagen and triple-helical (Pro-Pro-Gly)10 do not serve as substrate. 2-oxoadipate, 2-oxosuccinate or 2-oxovalerate do not replace 2-oxoglutarate Chlamydomonas reinhardtii ?
-
 ?
1.14.11.2 poly(L-Pro) + 2-oxoglutarate + O2
-
 Chlamydomonas reinhardtii poly(4-hydroxyproline) + succinate + CO2
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.11.2 30
-
-
 Chlamydomonas reinhardtii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.11.2 6.8 6.9
-
 Chlamydomonas reinhardtii

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.11.2 5,6-Isopropylideneascorbate can replace ascorbate Chlamydomonas reinhardtii
1.14.11.2 ascorbate Km: 0.240 mM Chlamydomonas reinhardtii
1.14.11.2 D-isoascorbate can replace ascorbate Chlamydomonas reinhardtii

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.14.11.2 0.008
-
 Pyridine 2,4-dicarboxylate
-
 Chlamydomonas reinhardtii
1.14.11.2 0.02
-
 Pyridine 2,5-dicarboxylate
-
 Chlamydomonas reinhardtii
1.14.11.2 0.05
-
 pyridine 2-carboxylate
-
 Chlamydomonas reinhardtii
1.14.11.2 0.07
-
 3,4-dihydroxybenzoate
-
 Chlamydomonas reinhardtii
1.14.11.2 0.2
-
 2-oxoadipinate
-
 Chlamydomonas reinhardtii
1.14.11.2 0.4
-
 2-oxosuccinate
-
 Chlamydomonas reinhardtii
1.14.11.2 1.5
-
 Glutarate
-
 Chlamydomonas reinhardtii
1.14.11.2 1.6
-
 succinate
-
 Chlamydomonas reinhardtii
1.14.11.2 11
-
 Benzene 1,3-dicarboxylate
-
 Chlamydomonas reinhardtii
1.14.11.2 20
-
 pyruvate
-
 Chlamydomonas reinhardtii