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Literature summary extracted from

  • Majamaa, K.; Hanauske-Abel, H.M.; Gunzler, V.; Kivirikko, K.I.
    The 2-oxoglutarate binding site of prolyl 4-hydroxylase. Identification of distinct subsites and evidence for 2-oxoglutarate decarboxylation in a ligand reaction at the enzyme-bound ferrous ion (1984), Eur. J. Biochem., 138, 239-245.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.11.2 bovine serum albumin activation Gallus gallus
1.14.11.2 catalase activation Gallus gallus
1.14.11.2 dithiothreitol activation Gallus gallus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.11.2 2-oxoadipinate competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ Gallus gallus
1.14.11.2 2-oxobutyrate competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ Gallus gallus
1.14.11.2 2-oxosuccinate competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+; i.e. oxaloacetate Gallus gallus
1.14.11.2 2-oxovalerate competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ Gallus gallus
1.14.11.2 3-oxoglutarate competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ Gallus gallus
1.14.11.2 adipinate competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ Gallus gallus
1.14.11.2 benzene 1,2-dicarboxylate competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ Gallus gallus
1.14.11.2 Benzene 1,3-dicarboxylate competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ Gallus gallus
1.14.11.2 Benzene 1,4-dicarboxylate competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ Gallus gallus
1.14.11.2 benzoate competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ Gallus gallus
1.14.11.2 Glutarate competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ Gallus gallus
1.14.11.2 levulinate competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ Gallus gallus
1.14.11.2 malonate competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ Gallus gallus
1.14.11.2 pyridine 2,3-dicarboxylate competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ Gallus gallus
1.14.11.2 Pyridine 2,4-dicarboxylate competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ Gallus gallus
1.14.11.2 Pyridine 2,5-dicarboxylate competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ Gallus gallus
1.14.11.2 pyridine 2,6-dicarboxylate competitive with respect to Fe2+ and noncompetitive with respect to 2-oxoglutarate Gallus gallus
1.14.11.2 pyridine 2-carboxylate competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ Gallus gallus
1.14.11.2 Pyridine 3,4-dicarboxylate competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ Gallus gallus
1.14.11.2 Pyridine 3,5-dicarboxylate competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ Gallus gallus
1.14.11.2 pyridine 3-carboxylate competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ Gallus gallus
1.14.11.2 Pyridine 4-carboxylate competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ Gallus gallus
1.14.11.2 pyruvate competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ Gallus gallus
1.14.11.2 succinate competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ Gallus gallus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.11.2 0.14
-
 carboxymethylated collagen L-proline as cosubstrate Gallus gallus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.2 Fe2+
-
 Gallus gallus

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.2 Gallus gallus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.11.2 embryo
-
 Gallus gallus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.2 (Pro-Pro-Gly)n + 2-oxoglutarate + O2 n: 1,5,10 Gallus gallus (Pro-4-hydroxy-Pro-Gly)n + succinate + CO2 n: 1,5,10 ?
1.14.11.2 carboxymethylated collagen L-proline + 2-oxoglutarate + O2
-
 Gallus gallus carboxymethylated collagen-trans-4-hydroxy-L-proline + succinate + CO2
-
 r
1.14.11.2 additional information thermal denaturing of the triple-helical conformation of the substrate before hydroxylation Gallus gallus ?
-
 ?
1.14.11.2 additional information characterization of the 2-oxoglutarate binding site of the enzyme, 2-oxosuccinate, 2-oxovalerate, 2-oxobutyrate, 3-oxoglutarate or pyridine 2,5-dicarboxylate do not replace 2-oxoglutarate as cosubstrates Gallus gallus ?
-
 ?
1.14.11.2 protocollagen + 2-oxoglutarate + O2 2-oxoadipate can replace 2-oxoglutarate as cosubstrate Gallus gallus 4-hydroxyproline containing protocollagen + succinate + CO2
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.11.2 37
-
 assay at Gallus gallus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.11.2 7.8
-
 assay at Gallus gallus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.11.2 ascorbate
-
 Gallus gallus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.14.11.2 0.0008
-
 Pyridine-2,5-dicarboxylate
-
 Gallus gallus
1.14.11.2 0.002
-
 Pyridine-2,4-dicarboxylate
-
 Gallus gallus
1.14.11.2 0.025
-
 pyridine 2-carboxylate
-
 Gallus gallus
1.14.11.2 0.1
-
 2-oxosuccinate
-
 Gallus gallus
1.14.11.2 0.21
-
 pyridine 2,3-dicarboxylate
-
 Gallus gallus
1.14.11.2 0.4
-
 succinate
-
 Gallus gallus
1.14.11.2 0.5
-
 pyridine 3-carboxylate
-
 Gallus gallus
1.14.11.2 0.8
-
 3-oxoglutarate
-
 Gallus gallus
1.14.11.2 0.9
-
 2-oxoadipinate
-
 Gallus gallus
1.14.11.2 0.9
-
 Pyridine 3,5-dicarboxylate
-
 Gallus gallus
1.14.11.2 0.9
-
 Pyridine 4-carboxylate
-
 Gallus gallus
1.14.11.2 2.2
-
 Pyridine 3,4-dicarboxylate
-
 Gallus gallus
1.14.11.2 3.3
-
 Benzene 1,4-dicarboxylate
-
 Gallus gallus
1.14.11.2 4.2
-
 Glutarate
-
 Gallus gallus
1.14.11.2 5.8
-
 Benzene 1,3-dicarboxylate
-
 Gallus gallus
1.14.11.2 5.9
-
 malonate
-
 Gallus gallus
1.14.11.2 7.6
-
 2-oxobutyrate
-
 Gallus gallus
1.14.11.2 9.4
-
 2-oxovalerate
-
 Gallus gallus
1.14.11.2 9.9
-
 benzoate
-
 Gallus gallus
1.14.11.2 10
-
 benzene 1,2-dicarboxylate value above Gallus gallus
1.14.11.2 15
-
 pyruvate
-
 Gallus gallus
1.14.11.2 15
-
 levulinate value above Gallus gallus
1.14.11.2 15
-
 adipinate value above Gallus gallus