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Literature summary extracted from

  • De Jong, L.; Kemp, A.
    Stoicheiometry and kinetics of the prolyl 4-hydroxylase partial reaction (1984), Biochim. Biophys. Acta, 787, 105-111.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.11.2 bovine serum albumin activation Gallus gallus
1.14.11.2 catalase activation Gallus gallus
1.14.11.2 dithiothreitol activation Gallus gallus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.11.2 Pyridine 2,4-dicarboxylate
-
 Gallus gallus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.11.2 0.02
-
 2-oxoglutarate partial reaction or complete reaction with in the presence of (Pro-Pro-Gly)n as a peptide substrate Gallus gallus
1.14.11.2 0.19
-
 O2 complete reaction with (Pro-Pro-Gly)5 as a peptide substrate Gallus gallus
1.14.11.2 1.5
-
 O2 partial reaction Gallus gallus
1.14.11.2 1.8
-
 (L-Pro-L-Pro-Gly)5 O2 with cosubstrate polyprolin Gallus gallus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.2 Fe2+
-
 Gallus gallus

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.2 Gallus gallus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.11.2 embryo
-
 Gallus gallus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.2 (L-Pro-L-Pro-Gly)5 + 2-oxoglutarate + O2
-
 Gallus gallus (L-Pro-L-Pro-Gly)5-trans-4-hydroxy-L-proline + succinate + CO2
-
 r
1.14.11.2 (Pro-Pro-Gly)n + 2-oxoglutarate + O2 n: 1,5,10 Gallus gallus (Pro-4-hydroxy-Pro-Gly)n + succinate + CO2 n: 1,5,10 ?
1.14.11.2 2-oxoglutarate + O2 + ascorbate uncoupled oxidative decarboxylation Gallus gallus succinate + dehydroascorbate + CO2 + H2O
-
 ?
1.14.11.2 additional information in the absence of a peptidylproline substrate, the oxidative decarboxylation of 2-oxoglutarate by the enzyme is stoichiometrically coupled to the oxidation of ascorbate Gallus gallus ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.11.2 37
-
 assay at Gallus gallus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.11.2 7.7
-
 assay at Gallus gallus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.11.2 ascorbate
-
 Gallus gallus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.14.11.2 0.002
-
 Pyridine 2,4-dicarboxylate
-
 Gallus gallus