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Literature summary extracted from

  • Gunzler, V.; Hanauske-Abel, H.M.; Myllylä, R.; Mohr, J.; Kivirikko, K.I.
    Time-dependent inactivation of chick-embryo prolyl 4-hydroxylase by coumalic acid. Evidence for a syncatalytic mechanism (1987), Biochem. J., 242, 163-169.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.11.2 bovine serum albumin activation Gallus gallus
1.14.11.2 bovine serum albumin activation Homo sapiens
1.14.11.2 catalase activation Gallus gallus
1.14.11.2 catalase activation Homo sapiens
1.14.11.2 dithiothreitol activation Gallus gallus
1.14.11.2 dithiothreitol activation Homo sapiens

General Stability

EC Number General Stability Organism
1.14.11.2 unstable in absence of 2-oxoglutarate and either Fe2+ or ascorbate Gallus gallus
1.14.11.2 unstable in absence of 2-oxoglutarate and either Fe2+ or ascorbate Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.11.2 3,4-dihydroxybenzoate
-
 Gallus gallus
1.14.11.2 3,4-dihydroxybenzoate
-
 Homo sapiens
1.14.11.2 4-hydroxybenzoate
-
 Gallus gallus
1.14.11.2 4-hydroxybenzoate
-
 Homo sapiens
1.14.11.2 Coumalic acid competitive inhibitor, potential syncatalytic inhibitor, time-dependent inactivation, increasing concentrations of Fe2+ enhance the inactivation; i.e. 2-oxo-1,2H-pyran-5-carboxylic acid Gallus gallus
1.14.11.2 Coumalic acid competitive inhibitor, potential syncatalytic inhibitor, time-dependent inactivation, increasing concentrations of Fe2+ enhance the inactivation; i.e. 2-oxo-1,2H-pyran-5-carboxylic acid Homo sapiens
1.14.11.2 Pyridine 2,4-dicarboxylate
-
 Gallus gallus
1.14.11.2 Pyridine 2,4-dicarboxylate
-
 Homo sapiens
1.14.11.2 Pyridine 2,5-dicarboxylate
-
 Gallus gallus
1.14.11.2 Pyridine 2,5-dicarboxylate
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.11.2 0.022
-
 2-oxoglutarate
-
 Gallus gallus
1.14.11.2 0.022
-
 2-oxoglutarate
-
 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.2 Gallus gallus
-
-
-
1.14.11.2 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.11.2 using ammonium sulfate fractionation, affinity chromatography on poly(L-proline) coupled to Sepharose 4B, DEAE-cellulose chromatography, and gel filtration Gallus gallus
1.14.11.2 using ammonium sulfate fractionation, affinity chromatography on poly(L-proline) coupled to Sepharose 4B, DEAE-cellulose chromatography, and gel filtration Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.11.2 embryo
-
 Gallus gallus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.2 (Pro-Pro-Gly)n + 2-oxoglutarate + O2 n: 1,5,10 Gallus gallus (Pro-4-hydroxy-Pro-Gly)n + succinate + CO2 n: 1,5,10 ?
1.14.11.2 (Pro-Pro-Gly)n + 2-oxoglutarate + O2 n: 1,5,10 Homo sapiens (Pro-4-hydroxy-Pro-Gly)n + succinate + CO2 n: 1,5,10 ?

Subunits

EC Number Subunits Comment Organism
1.14.11.2 tetramer the 2-oxoglutarate-binding site of the enzyme is located within the alpha-subunit Gallus gallus
1.14.11.2 tetramer the 2-oxoglutarate-binding site of the enzyme is located within the alpha-subunit Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.11.2 37
-
 assay at Gallus gallus
1.14.11.2 37
-
 assay at Homo sapiens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.14.11.2 7.5
-
 increased spontaneous inactivation at pH values above in the absence of an inactivating compound Gallus gallus
1.14.11.2 7.5
-
 increased spontaneous inactivation at pH values above in the absence of an inactivating compound Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.11.2 ascorbate
-
 Gallus gallus
1.14.11.2 ascorbate
-
 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.14.11.2 0.0008
-
 Pyridine-2,5-dicarboxylate
-
 Gallus gallus
1.14.11.2 0.0008
-
 Pyridine-2,5-dicarboxylate
-
 Homo sapiens
1.14.11.2 0.002
-
 Pyridine-2,4-dicarboxylate
-
 Gallus gallus
1.14.11.2 0.002
-
 Pyridine-2,4-dicarboxylate
-
 Homo sapiens
1.14.11.2 0.005
-
 3,4-dihydroxybenzoate
-
 Gallus gallus
1.14.11.2 0.005
-
 3,4-dihydroxybenzoate
-
 Homo sapiens
1.14.11.2 3.2
-
 4-hydroxybenzoate
-
 Gallus gallus
1.14.11.2 3.2
-
 4-hydroxybenzoate
-
 Homo sapiens
1.14.11.2 17
-
 Coumalic acid S-parabolic competitive inhibition with respect to 2-oxoglutarate Gallus gallus
1.14.11.2 17
-
 Coumalic acid S-parabolic competitive inhibition with respect to 2-oxoglutarate Homo sapiens