Literature summary extracted from
Gibson, D.T.; Resnick, S.M.; Lee, K.; Brand, J.M.; Torok, D.S.; Wackett, L.P.; Schocken, M.J.; Haigler, B.E.
Desaturation, dioxygenation, and monooxygenation reactions catalyzed by naphthalene dioxygenase from Pseudomonas sp. strain 9816-4 (1995), J. Bacteriol., 177, 2615-2621.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.14.12.12 |
expression of ferredoxinNAP reductase, ferredoxinNAP and ISPNAP in Escherichia coli JM 109 |
Pseudomonas sp. |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.14.12.12 |
Pseudomonas sp. |
- |
strain NCIB 9816/11, naphthalene dihydrogenase mutant derived from strain NCIB 9816-4 oxidizing naphthalene to cis-(1R,2S)-dihydroxy-1,2-dihydronaphthalene |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.14.12.12 |
(1S)-indanol + NADH + O2 |
recombinant enzyme |
Pseudomonas sp. |
(1S)-indanol + trans-1,3-indandiol |
85.5% indanol, 11.5% 1,3-indandiol, minor products: (1S)-indenol and 1-indanone |
? |
|
1.14.12.12 |
indan + NADH + H+ + O2 |
recombinant enzyme, enantiospecific monooxygenation |
Pseudomonas sp. |
(1S)-indenol + (1S)-indanol + NAD+ |
20% indenol, 67.1% indanol, minor products: indene, 1-indanone and (1R,2S)-indandiol |
? |
|
1.14.12.12 |
indene + NADH + O2 |
recombinant enzyme |
Pseudomonas sp. |
(1S)-indenol + (1R,2S)-indandiol + NAD+ |
57.9% indenol, 29.6% indandiol |
? |
|