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Literature summary extracted from

  • Suzuki, K.; Ogawa, N.; Miyashita, K.
    Expression of 2-halobenzoate dioxygenase genes (cbdSABC) involved in the degradation of benzoate and 2-halobenzoate in Burkholderia sp. TH2 (2001), Gene, 262, 137-145.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.12.13 several constructs with relevant genes are expressed in Escherichia coli DH5alpha and S17-1 lambdapir Burkholderia sp.

Protein Variants

EC Number Protein Variants Comment Organism
1.14.12.13 additional information interposon mutagenesis, resulting strain TD2 fails to grow on benzoate or 2-chlorobenzoate showing that the cbd gene is involved in their degradation Burkholderia sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.12.13 2-chlorobenzoate + NADH + H+ + O2 Burkholderia sp.
-
 catechol + chloride + NAD+ + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.13 Burkholderia sp.
-
 TH2, isolated from soil
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.12.13 0.006
-
 with 4-chlorobenzoate as substrate Burkholderia sp.
1.14.12.13 0.032
-
 with 2-iodobenzoate as substrate Burkholderia sp.
1.14.12.13 0.073
-
 with 3-chlorobenzoate as substrate Burkholderia sp.
1.14.12.13 0.195
-
 with benzoate as substrate Burkholderia sp.
1.14.12.13 0.303
-
 with 2-bromobenzoate as substrate Burkholderia sp.
1.14.12.13 0.458
-
 with 2-chlorobenzoate as substrate Burkholderia sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.13 2-bromobenzoate + NADH + O2
-
 Burkholderia sp. catechol + bromide + NAD+ + CO2
-
 ?
1.14.12.13 2-chlorobenzoate + NADH + H+ + O2
-
 Burkholderia sp. catechol + chloride + NAD+ + CO2
-
 ?
1.14.12.13 2-iodobenzoate + NADH + O2
-
 Burkholderia sp. catechol + iodide + NAD+ + CO2
-
 ?
1.14.12.13 2-methylbenzoate + NADH + O2 named o-toluate Burkholderia sp. o-cresol + ? + NAD+ + CO2
-
 ?
1.14.12.13 3-chlorobenzoate + NADH + O2
-
 Burkholderia sp. ? + NAD+ + CO2
-
 ?
1.14.12.13 benzoate + NADH + H+ + O2
-
 Burkholderia sp. catechol + NAD+ + CO2
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.12.13 30
-
 assay at, assay with cells Burkholderia sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.12.13 7.4
-
 assay at, assay with cells Burkholderia sp.