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Literature summary extracted from

  • Haak, B.; Fetzner, S.; Lingens, F.
    Cloning, nucleotide sequence, and expression of the plasmid-encoded genes for the two-component 2-halobenzoate 1,2-dioxygenase from Pseudomonas cepacia 2CBS (1995), J. Bacteriol., 177, 667-675.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.12.13 Escherichia coli TG1, JM105, MV1190 and Pseudomonas putida KT2440 are used as host strains Burkholderia cepacia

Protein Variants

EC Number Protein Variants Comment Organism
1.14.12.13 additional information several mutants of Pseudomonas cepacia, putida and sp. are used Burkholderia cepacia

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.12.13 Fe2+ iron-sulfur protein Burkholderia cepacia
1.14.12.13 Sulfide iron-sulfur protein Burkholderia cepacia

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.12.13 19500
-
 x * 19500, x * 52400, alpha3beta3, small and large subunit of the oxygenase component, SDS-PAGE Burkholderia cepacia
1.14.12.13 37100
-
 1 * 37100, NADH:acceptor reductase component, SDS-PAGE Burkholderia cepacia
1.14.12.13 52400
-
 x * 19500, x * 52400, alpha3beta3, small and large subunit of the oxygenase component, SDS-PAGE Burkholderia cepacia

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.12.13 2-chlorobenzoate + NADH + H+ + O2 Burkholderia cepacia initial step in the degradation of aromatic compounds catechol + chloride + NAD+ + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.13 Burkholderia cepacia
-
 2CBS, isolated from water
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.12.13 anion-exchange Burkholderia cepacia

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.12.13 2-chlorobenzoate + NADH + H+ + O2 = catechol + chloride + NAD+ + CO2 two-component nonheme iron dioxygenase system of oxygenase system class IB dioxygenase: NADH:acceptor reductase containing both FAD and cloroplast-type 2Fe-2S and Rieske-type 2Fe-2S-containing oxygenase component Burkholderia cepacia
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.13 2-bromobenzoate + NADH + O2
-
 Burkholderia cepacia catechol + bromide + NAD+ + CO2
-
 ?
1.14.12.13 2-chlorobenzoate + NADH + H+ + O2
-
 Burkholderia cepacia catechol + chloride + NAD+ + CO2
-
 ?
1.14.12.13 2-chlorobenzoate + NADH + H+ + O2 initial step in the degradation of aromatic compounds Burkholderia cepacia catechol + chloride + NAD+ + CO2
-
 ?
1.14.12.13 2-fluorobenzoate + NADH + O2
-
 Burkholderia cepacia catechol + fluoride + NAD+ + CO2
-
 ?
1.14.12.13 2-iodobenzoate + NADH + O2
-
 Burkholderia cepacia catechol + iodide + NAD+ + CO2
-
 ?
1.14.12.13 additional information very broad substrate specificity Burkholderia cepacia ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.12.13 monomer 1 * 37100, NADH:acceptor reductase component, SDS-PAGE Burkholderia cepacia
1.14.12.13 oligomer x * 19500, x * 52400, alpha3beta3, small and large subunit of the oxygenase component, SDS-PAGE Burkholderia cepacia

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.12.13 FAD
-
 Burkholderia cepacia
1.14.12.13 NADH
-
 Burkholderia cepacia