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Literature summary extracted from

  • Swift, R.J.; Carter, S.F.; Widdowson, D.A.; Mason, J.R.; Leak, D.J.
    Expression of benzene dioxygenase from Pseudomonas putida ML2 in cis-1,2-cyclohexanediol-degrading pseudomonads (2001), Appl. Microbiol. Biotechnol., 55, 721-726.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.12.3 expression in Escherichia coli JM109 Pseudomonas putida
1.14.12.3 genes bedC1C2BA, under the control of the tac promoter, subcloned into pLAFR5, successfully conjugated into seven of the Gram-negative cis-1,2-cyclohexanediol-degrading isolates and stably maintained and expressed in three of them, strain CHD1–CHD3, the strains grow on cis-1,2-cyclohexanediol as sole carbon source, express an active BDO and oxidise cyclohexene, but none of the three strains is able to grow on cyclohexene as sole carbon source, overview, expression in Escherichia coli strain JM109 Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.12.3 Fe2+ required Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.12.3 1-methylcyclohexene + O2 + NADH Pseudomonas putida
-
 ?
-
 ?
1.14.12.3 1-methylcyclohexene + O2 + NADH Pseudomonas putida ML2
-
 ?
-
 ?
1.14.12.3 2 cyclohexene + 2 O2 + 2 NADH + 2 H+ Pseudomonas putida
-
 2-cyclohexen-1-ol + cis-1,2-cyclohexanediol + 2 NAD+ + H2O failure of transformed bacteria to grow on cyclohexene is attributed to the toxicity of metabolic intermediates accumulating from the 2-cyclohexen-1-ol metabolism, cyclohexene metabolism pathways, overview ?
1.14.12.3 3-methylcyclohexene + O2 + NADH Pseudomonas putida
-
 ?
-
 ?
1.14.12.3 3-methylcyclohexene + O2 + NADH Pseudomonas putida ML2
-
 ?
-
 ?
1.14.12.3 benzene + O2 + NADH Pseudomonas putida
-
 cis-cyclohexa-3,5-diene-1,2-diol + NAD+
-
 ?
1.14.12.3 benzene + O2 + NADH Pseudomonas putida ML2
-
 cis-cyclohexa-3,5-diene-1,2-diol + NAD+
-
 ?
1.14.12.3 toluene + O2 + NADH Pseudomonas putida
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.3 Pseudomonas putida
-
-
-
1.14.12.3 Pseudomonas putida
-
 gene bedC1C2BA
-
1.14.12.3 Pseudomonas putida ML2
-
-
-
1.14.12.3 Pseudomonas putida ML2
-
 gene bedC1C2BA
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.12.3 0.0049
-
 recombinant enzyme expressed in Escherichia coli, substrate cyclohexene Pseudomonas putida
1.14.12.3 0.0077
-
 recombinant enzyme expressed in Escherichia coli, substrate 1-methylcyclohexene Pseudomonas putida
1.14.12.3 0.0086
-
 recombinant enzyme expressed in Escherichia coli, substrate 3-methylcyclohexene Pseudomonas putida
1.14.12.3 0.0172
-
 recombinant enzyme expressed in Escherichia coli, substrate toluene Pseudomonas putida
1.14.12.3 0.0236
-
 recombinant enzyme expressed in Escherichia coli, substrate benzene Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.3 1-methylcyclohexene + O2 + NADH
-
 Pseudomonas putida ?
-
 ?
1.14.12.3 1-methylcyclohexene + O2 + NADH
-
 Pseudomonas putida ML2 ?
-
 ?
1.14.12.3 2 cyclohexene + 2 O2 + 2 NADH + 2 H+
-
 Pseudomonas putida 2-cyclohexen-1-ol + cis-1,2-cyclohexanediol + 2 NAD+ + H2O
-
 ?
1.14.12.3 2 cyclohexene + 2 O2 + 2 NADH + 2 H+
-
 Pseudomonas putida 2-cyclohexen-1-ol + cis-1,2-cyclohexanediol + 2 NAD+ + H2O failure of transformed bacteria to grow on cyclohexene is attributed to the toxicity of metabolic intermediates accumulating from the 2-cyclohexen-1-ol metabolism, cyclohexene metabolism pathways, overview ?
1.14.12.3 3-methylcyclohexene + O2 + NADH
-
 Pseudomonas putida ?
-
 ?
1.14.12.3 3-methylcyclohexene + O2 + NADH
-
 Pseudomonas putida ML2 ?
-
 ?
1.14.12.3 benzene + NADH + O2
-
 Pseudomonas putida cis-cyclohexa-3,5-diene-1,2-diol + NAD+ the enzyme oxidizes cyclohexene to a mixture of two products, a monohydroxylated 2-cyclohexen-1-ol product and a dihydroxylated cis-1,2-cyclohexanediol ?
1.14.12.3 benzene + NADH + O2
-
 Pseudomonas putida ML2 cis-cyclohexa-3,5-diene-1,2-diol + NAD+ the enzyme oxidizes cyclohexene to a mixture of two products, a monohydroxylated 2-cyclohexen-1-ol product and a dihydroxylated cis-1,2-cyclohexanediol ?
1.14.12.3 benzene + O2 + NADH
-
 Pseudomonas putida cis-cyclohexa-3,5-diene-1,2-diol + NAD+
-
 ?
1.14.12.3 benzene + O2 + NADH best substrate Pseudomonas putida cis-cyclohexa-3,5-diene-1,2-diol + NAD+
-
 ?
1.14.12.3 benzene + O2 + NADH
-
 Pseudomonas putida ML2 cis-cyclohexa-3,5-diene-1,2-diol + NAD+
-
 ?
1.14.12.3 benzene + O2 + NADH best substrate Pseudomonas putida ML2 cis-cyclohexa-3,5-diene-1,2-diol + NAD+
-
 ?
1.14.12.3 toluene + O2 + NADH
-
 Pseudomonas putida ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.12.3 BDO
-
 Pseudomonas putida
1.14.12.3 benzene dioxygenase
-
 Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.12.3 30
-
 assay at Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.12.3 7.2
-
 assay at Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.12.3 NADH
-
 Pseudomonas putida