EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.14.13.241 | - |
Pseudomonas sp. |
1.14.13.242 | - |
Pseudomonas sp. |
EC Number | General Stability | Organism |
---|---|---|
1.14.13.241 | activity is lost on resolution with acidic ammonium sulfate and can be completely restored with FAD+, but not with FMN | Pseudomonas sp. |
1.14.13.241 | highly sensitive to sulfhydryl reagents but not to chelating agents and is stabilized by high concentrations of mercaptoethanol and glycerol | Pseudomonas sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.241 | 5-pyridoxic acid | - |
Pseudomonas sp. | |
1.14.13.241 | 6-methylnicotinic acid | - |
Pseudomonas sp. | |
1.14.13.241 | p-chloromercuribenzoate | - |
Pseudomonas sp. | |
1.14.13.242 | 5-pyridoxic acid | competitive with 3-hydroxy-2-methylpyridine-5-carboxylate | Pseudomonas sp. | |
1.14.13.242 | 6-Methylnicotinate | - |
Pseudomonas sp. | |
1.14.13.242 | 6-methylnicotinic acid | competitive with 3-hydroxy-2-methylpyridine-5-carboxylate | Pseudomonas sp. | |
1.14.13.242 | p-chloromercuribenzoate | 0.05 mM, quick and complete inhibition | Pseudomonas sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.13.241 | 166000 | - |
sedimentation equlibrium | Pseudomonas sp. |
1.14.13.242 | 166000 | - |
equilibrium sedimentation | Pseudomonas sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.241 | 3-hydroxy-4-hydroxymethyl-2-methylpyridine-5-carboxylate + NADPH + O2 + H3O+ | Pseudomonas sp. | bacterial vitamin B6 degradation pathway | 2-(acetamidomethylene)-3-(hydroxymethyl)succinate + NADP+ + H2O | - |
? | |
1.14.13.242 | 3-hydroxy-2-methylpyridine-5-carboxylate + NADH + O2 | Pseudomonas sp. | inducible enzyme opens the pyridine ring during the metabolic degradation of vitamin B6 | 2-(acetamidomethylene)succinate + NAD+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.13.241 | Pseudomonas sp. | - |
- |
- |
1.14.13.242 | Pseudomonas sp. | - |
MA-1 | - |
EC Number | Oxidation Stability | Organism |
---|---|---|
1.14.13.241 | highly sensitive to oxidation | Pseudomonas sp. |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.13.241 | - |
Pseudomonas sp. |
1.14.13.242 | - |
Pseudomonas sp. |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.14.13.242 | 3-hydroxy-2-methylpyridine-5-carboxylate + NAD(P)H + H+ + O2 = 2-(acetamidomethylidene)succinate + NAD(P)+ | reaction proceeds in a concerted fashion via a ternary complex of oxygenase, NADH and 3-hydroxy-2-methylpyridine-5-carboxylate | Pseudomonas sp. |
EC Number | Storage Stability | Organism |
---|---|---|
1.14.13.241 | -20°C, can be stored up to 1 month with loss about 20% of its activity in a mixture of 50% glycerol and 0.1% 2-mercaptoethanol | Pseudomonas sp. |
1.14.13.241 | 4°C, loses activity rapidly in absence of mercaptoethanol | Pseudomonas sp. |
1.14.13.242 | -20°C, 50% glycerol, 0.1% 2-mercaptoethanol, 1 month | Pseudomonas sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.241 | 3-hydroxy-4-hydroxymethyl-2-methylpyridine-5-carboxylate + NADPH + O2 | - |
Pseudomonas sp. | 2-(acetamidomethylene)-3-(hydroxymethyl)succinate + NADP+ + H2O | - |
? | |
1.14.13.241 | 3-hydroxy-4-hydroxymethyl-2-methylpyridine-5-carboxylate + NADPH + O2 + H3O+ | bacterial vitamin B6 degradation pathway | Pseudomonas sp. | 2-(acetamidomethylene)-3-(hydroxymethyl)succinate + NADP+ + H2O | - |
? | |
1.14.13.241 | 5-pyridoxic acid + NADPH + O2 + H3O+ | reaction rate 3% | Pseudomonas sp. | 2-(acetylaminomethylene)succinate + NADP+ + H2O | - |
? | |
1.14.13.241 | additional information | 2,6-dichloroindophenol is no substrate | Pseudomonas sp. | ? | - |
? | |
1.14.13.242 | 3-hydroxy-2-methylpyridine-5-carboxylate + NADH + O2 | - |
Pseudomonas sp. | 2-(acetamidomethylene)succinate + NAD(P)+ | - |
? | |
1.14.13.242 | 3-hydroxy-2-methylpyridine-5-carboxylate + NADH + O2 | inducible enzyme opens the pyridine ring during the metabolic degradation of vitamin B6 | Pseudomonas sp. | 2-(acetamidomethylene)succinate + NAD+ | - |
? | |
1.14.13.242 | 5-pyridoxic acid + NADH + O2 | - |
Pseudomonas sp. | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.13.241 | compound I oxygenase | - |
Pseudomonas sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.13.241 | 6.5 | 8 | broad pH-optimum in phosphate, diphosphate and Tris buffer | Pseudomonas sp. |
1.14.13.242 | 6.5 | 8 | - |
Pseudomonas sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.241 | FAD | contains 2 mol of FAD+ per mol | Pseudomonas sp. | |
1.14.13.242 | FAD | contains 2 mol of FAD per mol of enzyme | Pseudomonas sp. | |
1.14.13.242 | NADH | - |
Pseudomonas sp. |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.13.241 | 0.06 | - |
5-pyridoxic acid | - |
Pseudomonas sp. | |
1.14.13.241 | 0.2 | - |
6-methylnicotinic acid | - |
Pseudomonas sp. |