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Literature summary extracted from

  • Sparrow, L.G.; Ho, P.P.K.; Sundaram, T.K.; Zach, D.; Nyns, E.J.; Snell, E.E.
    The bacterial oxidation of vitamin B6. VII. Purification, properties, and mechanism of action of an oxygenase which cleaves the 3-hydroxypyridine ring (1969), J. Biol. Chem., 244, 2590-2600.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.13.241
-
Pseudomonas sp.
1.14.13.242
-
Pseudomonas sp.

General Stability

EC Number General Stability Organism
1.14.13.241 activity is lost on resolution with acidic ammonium sulfate and can be completely restored with FAD+, but not with FMN Pseudomonas sp.
1.14.13.241 highly sensitive to sulfhydryl reagents but not to chelating agents and is stabilized by high concentrations of mercaptoethanol and glycerol Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.241 5-pyridoxic acid
-
Pseudomonas sp.
1.14.13.241 6-methylnicotinic acid
-
Pseudomonas sp.
1.14.13.241 p-chloromercuribenzoate
-
Pseudomonas sp.
1.14.13.242 5-pyridoxic acid competitive with 3-hydroxy-2-methylpyridine-5-carboxylate Pseudomonas sp.
1.14.13.242 6-Methylnicotinate
-
Pseudomonas sp.
1.14.13.242 6-methylnicotinic acid competitive with 3-hydroxy-2-methylpyridine-5-carboxylate Pseudomonas sp.
1.14.13.242 p-chloromercuribenzoate 0.05 mM, quick and complete inhibition Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.241 166000
-
sedimentation equlibrium Pseudomonas sp.
1.14.13.242 166000
-
equilibrium sedimentation Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.241 3-hydroxy-4-hydroxymethyl-2-methylpyridine-5-carboxylate + NADPH + O2 + H3O+ Pseudomonas sp. bacterial vitamin B6 degradation pathway 2-(acetamidomethylene)-3-(hydroxymethyl)succinate + NADP+ + H2O
-
?
1.14.13.242 3-hydroxy-2-methylpyridine-5-carboxylate + NADH + O2 Pseudomonas sp. inducible enzyme opens the pyridine ring during the metabolic degradation of vitamin B6 2-(acetamidomethylene)succinate + NAD+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.241 Pseudomonas sp.
-
-
-
1.14.13.242 Pseudomonas sp.
-
MA-1
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.14.13.241 highly sensitive to oxidation Pseudomonas sp.

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.241
-
Pseudomonas sp.
1.14.13.242
-
Pseudomonas sp.

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.13.242 3-hydroxy-2-methylpyridine-5-carboxylate + NAD(P)H + H+ + O2 = 2-(acetamidomethylidene)succinate + NAD(P)+ reaction proceeds in a concerted fashion via a ternary complex of oxygenase, NADH and 3-hydroxy-2-methylpyridine-5-carboxylate Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
1.14.13.241 -20°C, can be stored up to 1 month with loss about 20% of its activity in a mixture of 50% glycerol and 0.1% 2-mercaptoethanol Pseudomonas sp.
1.14.13.241 4°C, loses activity rapidly in absence of mercaptoethanol Pseudomonas sp.
1.14.13.242 -20°C, 50% glycerol, 0.1% 2-mercaptoethanol, 1 month Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.241 3-hydroxy-4-hydroxymethyl-2-methylpyridine-5-carboxylate + NADPH + O2
-
Pseudomonas sp. 2-(acetamidomethylene)-3-(hydroxymethyl)succinate + NADP+ + H2O
-
?
1.14.13.241 3-hydroxy-4-hydroxymethyl-2-methylpyridine-5-carboxylate + NADPH + O2 + H3O+ bacterial vitamin B6 degradation pathway Pseudomonas sp. 2-(acetamidomethylene)-3-(hydroxymethyl)succinate + NADP+ + H2O
-
?
1.14.13.241 5-pyridoxic acid + NADPH + O2 + H3O+ reaction rate 3% Pseudomonas sp. 2-(acetylaminomethylene)succinate + NADP+ + H2O
-
?
1.14.13.241 additional information 2,6-dichloroindophenol is no substrate Pseudomonas sp. ?
-
?
1.14.13.242 3-hydroxy-2-methylpyridine-5-carboxylate + NADH + O2
-
Pseudomonas sp. 2-(acetamidomethylene)succinate + NAD(P)+
-
?
1.14.13.242 3-hydroxy-2-methylpyridine-5-carboxylate + NADH + O2 inducible enzyme opens the pyridine ring during the metabolic degradation of vitamin B6 Pseudomonas sp. 2-(acetamidomethylene)succinate + NAD+
-
?
1.14.13.242 5-pyridoxic acid + NADH + O2
-
Pseudomonas sp. ?
-
?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.241 compound I oxygenase
-
Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.241 6.5 8 broad pH-optimum in phosphate, diphosphate and Tris buffer Pseudomonas sp.
1.14.13.242 6.5 8
-
Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.241 FAD contains 2 mol of FAD+ per mol Pseudomonas sp.
1.14.13.242 FAD contains 2 mol of FAD per mol of enzyme Pseudomonas sp.
1.14.13.242 NADH
-
Pseudomonas sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.14.13.241 0.06
-
5-pyridoxic acid
-
Pseudomonas sp.
1.14.13.241 0.2
-
6-methylnicotinic acid
-
Pseudomonas sp.