EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.14.12.9 | Iron | component A is an iron-sulfur protein, contains 1.6-1.8 mol of iron per subunit and 1.6-1.9 mol of acid-labile sulfide | Pseudomonas sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.12.9 | 140000 | - |
dioxygenase component of 4-chlorophenylacetate 3,4-dioxygenase, gel filtration | Pseudomonas sp. |
1.14.12.9 | 144000 | - |
dioxygenase component of 4-chlorophenylacetate 3,4-dioxygenase, ultracentrifugation | Pseudomonas sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.12.9 | Pseudomonas sp. | - |
- |
- |
1.14.12.9 | Pseudomonas sp. CBS 3 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.12.9 | dioxygenase component | Pseudomonas sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.12.9 | 0.144 | - |
dioxygenase component of of 4-chlorophenylacetate 3,4-dioxygenase | Pseudomonas sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.12.9 | 3-chlorophenylacetate + NADH + O2 + H2O | 10% of the activity of 4-chlorophenylacetate | Pseudomonas sp. | 3,4-dihydroxyphenylacetate + Cl- + NAD+ | - |
? | |
1.14.12.9 | 3-chlorophenylacetate + NADH + O2 + H2O | 10% of the activity of 4-chlorophenylacetate | Pseudomonas sp. CBS 3 | 3,4-dihydroxyphenylacetate + Cl- + NAD+ | - |
? | |
1.14.12.9 | 4-bromophenylacetate + NADH + O2 + H2O | 102% of the activity of 4-chlorophenylacetate | Pseudomonas sp. | 3,4-dihydroxyphenylacetate + Br- + NAD+ | - |
? | |
1.14.12.9 | 4-bromophenylacetate + NADH + O2 + H2O | 102% of the activity of 4-chlorophenylacetate | Pseudomonas sp. CBS 3 | 3,4-dihydroxyphenylacetate + Br- + NAD+ | - |
? | |
1.14.12.9 | 4-chlorophenylacetate + NADH + O2 + H2O | - |
Pseudomonas sp. | 3,4-dihydroxyphenylacetate + Cl- + NAD(P)+ | - |
? | |
1.14.12.9 | 4-chlorophenylacetate + NADH + O2 + H2O | - |
Pseudomonas sp. CBS 3 | 3,4-dihydroxyphenylacetate + Cl- + NAD(P)+ | - |
? | |
1.14.12.9 | 4-fluorophenylacetate + NADH + O2 + H2O | 30% of the activity of 4-chlorophenylacetate | Pseudomonas sp. | 3,4-dihydroxyphenylacetate + F- + NAD+ | - |
? | |
1.14.12.9 | 4-fluorophenylacetate + NADH + O2 + H2O | 30% of the activity of 4-chlorophenylacetate | Pseudomonas sp. CBS 3 | 3,4-dihydroxyphenylacetate + F- + NAD+ | - |
? | |
1.14.12.9 | 4-hydroxyphenylacetate + NADH + O2 + H2O | 4% of the activity of 4-chlorophenylacetate | Pseudomonas sp. | 3,4-dihydroxyphenylacetate + OH- + NAD+ | - |
? | |
1.14.12.9 | additional information | not active as substrate: benzoate, substituted analogues of benzoate, 2-chlorophenylacetate, antipyrin, 4-chlorocinnamate | Pseudomonas sp. | ? | - |
? | |
1.14.12.9 | additional information | not active as substrate: benzoate, substituted analogues of benzoate, 2-chlorophenylacetate, antipyrin, 4-chlorocinnamate | Pseudomonas sp. CBS 3 | ? | - |
? | |
1.14.12.9 | phenylacetate + NADH + O2 + H2O | 6% of the activity of 4-chlorophenylacetate | Pseudomonas sp. | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.12.9 | More | the enzyme consists of two components, a monomeric reductase and a trimeric dioxygenase | Pseudomonas sp. |
1.14.12.9 | trimer | 3 * 46000-52000, dioxygenase component of of 4-chlorophenylacetate 3,4-dioxygenase, SDS-PAGE | Pseudomonas sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.12.9 | NADH | - |
Pseudomonas sp. |