Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Beadle, C.A.; Smith, A.R.W.
    The purification and properties of 2,4-dichlorophenol hydroxylase from a strain of Acinetobacter species (1982), Eur. J. Biochem., 123, 323-332.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.14.13.20 2,4-dichlorophenol, 0.1 mM, stabilizes during purification Acinetobacter sp.
1.14.13.20 dithiothreitol, 1 mM, stabilizes during purification Acinetobacter sp.
1.14.13.20 EDTA, 0.1 mM, stabilizes during purification Acinetobacter sp.
1.14.13.20 FAD, 0.01 mM, stabilizes during purification Acinetobacter sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.20 2,4-Dichlorophenol above 1 mM Acinetobacter sp.
1.14.13.20 AgNO3
-
 Acinetobacter sp.
1.14.13.20 CuSO4
-
 Acinetobacter sp.
1.14.13.20 diethyldithiocarbamate
-
 Acinetobacter sp.
1.14.13.20 dithiothreitol slight Acinetobacter sp.
1.14.13.20 FeCl3
-
 Acinetobacter sp.
1.14.13.20 FeSO4
-
 Acinetobacter sp.
1.14.13.20 Hg2+ HgCl2 Acinetobacter sp.
1.14.13.20 KCN
-
 Acinetobacter sp.
1.14.13.20 p-chloromercuribenzoate
-
 Acinetobacter sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.20 0.004
-
 2,4-Dichlorophenol cosubstrate NADPH Acinetobacter sp.
1.14.13.20 0.057
-
 O2
-
 Acinetobacter sp.
1.14.13.20 0.083
-
 NADPH
-
 Acinetobacter sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.20 63000
-
 alpha4, 4 * 63000, SDS-PAGE Acinetobacter sp.
1.14.13.20 240000
-
 gel filtration Acinetobacter sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.20 2,4-dichlorophenol + NADPH + O2 Acinetobacter sp.
-
 3,5-dichlorocatechol + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.20 Acinetobacter sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.20
-
 Acinetobacter sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.20 1.46
-
-
 Acinetobacter sp.

Storage Stability

EC Number Storage Stability Organism
1.14.13.20 -20°C, 3 months stable Acinetobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.20 2,4-dichlorophenol + NADPH + O2
-
 Acinetobacter sp. 3,5-dichlorocatechol + NADP+ + H2O
-
 ?
1.14.13.20 2-chlorophenol + NADPH + O2 incompletely metabolized Acinetobacter sp. ?
-
 ?
1.14.13.20 2-cresol + NADPH + O2 incompletely metabolized Acinetobacter sp. ?
-
 ?
1.14.13.20 4-bromophenol + NADPH + O2 incompletely metabolized Acinetobacter sp. ?
-
 ?
1.14.13.20 4-chloro-2-methylphenol + NADPH + O2
-
 Acinetobacter sp. ?
-
 ?
1.14.13.20 4-chlorophenol + NADPH + O2
-
 Acinetobacter sp. ?
-
 ?
1.14.13.20 additional information pseudosubstrates evoke oxidation of NAD(P)H and oxygen consumption without themselves undergoing hydroxylation, the product is hydrogen peroxide: 2,5-dichlorophenol, 2,6-dichlorophenol, 3,4-dichlorophenol, 2, 4,5-trichlorophenol Acinetobacter sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.13.20 tetramer alpha4, 4 * 63000, SDS-PAGE Acinetobacter sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.20 29
-
-
 Acinetobacter sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.13.20 6.83
-
 2,4-Dichlorophenol
-
 Acinetobacter sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.20 7.6
-
-
 Acinetobacter sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.20 FAD flavoprotein Acinetobacter sp.
1.14.13.20 FAD FAD cannot be replaced by riboflavin or FMN Acinetobacter sp.
1.14.13.20 FAD 1 mol of enzyme contains: 1.9 mol FAD Acinetobacter sp.
1.14.13.20 NADH 5% of the activity with NADPH Acinetobacter sp.
1.14.13.20 NADPH
-
 Acinetobacter sp.