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Literature summary extracted from

  • Cirakoglu, B.; Waller, J.P.
    Leucyl-tRNA and lysyl-tRNA synthetases, derived from the high-Mr complex of sheep liver, are hydrophobic proteins (1985), Eur. J. Biochem., 151, 101-110.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.4 129000
-
 x * 129000, SDS-PAGE component of the high-MW multienzyme complex Ovis aries
6.1.1.4 150000
-
 HPLC gel filtration, component of the high-MW enzyme complex Ovis aries
6.1.1.6 79000
-
 2 * 79000, SDS-PAGE Ovis aries
6.1.1.6 150000
-
 HPLC gel filtration Ovis aries

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.4 Ovis aries
-
-
-
6.1.1.6 Ovis aries
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.4
-
 Ovis aries
6.1.1.6 from a high MW complex Ovis aries

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.1.1.4 liver
-
 Ovis aries
-
6.1.1.6 liver
-
 Ovis aries
-

Storage Stability

EC Number Storage Stability Organism
6.1.1.4 15°C, 0.1% Triton X-100, stable for several months Ovis aries

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.4 ATP + L-leucine + tRNALeu
-
 Ovis aries AMP + diphosphate + L-leucyl-tRNALeu
-
 ?
6.1.1.4 additional information leucine-dependent ATP-diphosphate exchange, leucine + ATP + enzyme/Ile-AMP-enzyme + diphosphate Ovis aries ?
-
 ?
6.1.1.6 ATP + lysine + tRNALys
-
 Ovis aries AMP + L-lysyl-tRNALys + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.4 ? x * 129000, SDS-PAGE component of the high-MW multienzyme complex Ovis aries
6.1.1.6 dimer 2 * 79000, SDS-PAGE Ovis aries