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Literature summary extracted from

  • Trower, M.K.; Buckland, R.M.; Griffin, M.
    Characterization of an FMN-containing cyclohexanone monooxygenase from a cyclohexane-grown Xanthobacter sp (1989), Eur. J. Biochem., 181, 199-206.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.22 5,5'-dithiobis(2-nitrobenzoate)
-
 Xanthobacter sp.
1.14.13.22 arsenate
-
 Xanthobacter sp.
1.14.13.22 CuCl2
-
 Xanthobacter sp.
1.14.13.22 FeCl3
-
 Xanthobacter sp.
1.14.13.22 p-hydroxymercuribenzoate
-
 Xanthobacter sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.22 0.0005
-
 cyclohexanone below Xanthobacter sp.
1.14.13.22 0.0125
-
 NADPH
-
 Xanthobacter sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.13.22 cytoplasm
-
 Xanthobacter sp. 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.22 50000
-
 gel filtration Xanthobacter sp.
1.14.13.22 51000
-
 1 * 51000, SDS-PAGE Xanthobacter sp.

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.22 Xanthobacter sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.22
-
 Xanthobacter sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.22 2.1
-
-
 Xanthobacter sp.

Storage Stability

EC Number Storage Stability Organism
1.14.13.22 -20°C, stable Xanthobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.22 2-hydroxycyclohexanone + NADPH + O2
-
 Xanthobacter sp. 1-oxa-2-oxo-3-hydroxycycloheptane + NADP+ + H2O
-
 ?
1.14.13.22 2-methylcyclohexanone + NADPH + O2
-
 Xanthobacter sp. 1-oxa-2-oxo-3-methylcycloheptane + NADP+ + H2O
-
 ?
1.14.13.22 cyclobutanone + NADPH + O2
-
 Xanthobacter sp. 1-oxa-2-oxo-cyclopentane + NADP+ + H2O
-
 ?
1.14.13.22 cycloheptanone + NADPH + O2
-
 Xanthobacter sp. 1-oxa-2-oxo-cyclooctane + NADP+ + H2O
-
 ?
1.14.13.22 cyclohexan-1,2-dione + NADPH + O2
-
 Xanthobacter sp. 1-oxa-2,3-dioxo-cycloheptane + NADP+ + H2O
-
 ?
1.14.13.22 cyclohexan-1,4-dione + NADPH + O2
-
 Xanthobacter sp. 1-oxa-2,5-dioxo-cycloheptane + NADP+ + H2O
-
 ?
1.14.13.22 cyclohexanone + NADPH + O2
-
 Xanthobacter sp. 6-hexanolide + NADP+ + H2O
-
 ?
1.14.13.22 cyclooctanone + NADPH + O2 low activity Xanthobacter sp. 1-oxa-2-oxo-cyclononane + NADP+ + H2O
-
 ?
1.14.13.22 cyclopentanone + NADPH + O2
-
 Xanthobacter sp. 1-oxa-2-oxo-cyclohexane + NADP+ + H2O
-
 ?
1.14.13.22 additional information no activity towards straight-chain alkanones Xanthobacter sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.13.22 monomer 1 * 51000, SDS-PAGE Xanthobacter sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.22 30
-
 assay at Xanthobacter sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.22 8.8
-
-
 Xanthobacter sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.13.22 6 11 at pH 6.0 and 11.0: about 10% of activity maximum Xanthobacter sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.22 FAD
-
 Xanthobacter sp.
1.14.13.22 FMN involved in catalytic mechanism Xanthobacter sp.
1.14.13.22 FMN 1 FMN molecule per molecule of protein Xanthobacter sp.
1.14.13.22 NADPH electron donor Xanthobacter sp.