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Literature summary extracted from
- m-Hydroxybenzoate 4-hydroxylase from Aspergillus niger: purification and properties (1973), Indian J. Biochem. Biophys., 10, 184-190.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.14.13.23 | 2-mercaptoethanol stabilizes | Aspergillus niger |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.23 | diethyldithiocarbamate | - |
Aspergillus niger |  |
| 1.14.13.23 | heavy metal ions | - |
Aspergillus niger | |
| 1.14.13.23 | m-Aminobenzoate | - |
Aspergillus niger | |
| 1.14.13.23 | N-ethylmaleimide | - |
Aspergillus niger | |
| 1.14.13.23 | o-phenanthroline | - |
Aspergillus niger | |
| 1.14.13.23 | p-hydroxymercuribenzoate | - |
Aspergillus niger | |
| 1.14.13.23 | Salicylaldoxime | - |
Aspergillus niger | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.13.23 | 0.19 | - |
3-hydroxybenzoate | - |
Aspergillus niger | |
| 1.14.13.23 | 0.2 | - |
NADPH | - |
Aspergillus niger | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.13.23 | 3-hydroxybenzoate + [reduced NADPH-hemoprotein reductase] + O2 | Aspergillus niger | - |
3,4-dihydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.13.23 | Aspergillus niger | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.13.23 | partial | Aspergillus niger |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.13.23 | mycelium | - |
Aspergillus niger | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.13.23 | 3-hydroxybenzoate + NADPH + H+ + O2 | - |
Aspergillus niger | 3,4-dihydroxybenzoate + NADP+ + H2O | - |
r | |
| 1.14.13.23 | 3-hydroxybenzoate + [reduced NADPH-hemoprotein reductase] + O2 | - |
Aspergillus niger | 3,4-dihydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.23 | 30 | - |
assay at | Aspergillus niger |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.23 | 7.2 | - |
- |
Aspergillus niger |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.23 | FAD | flavoprotein | Aspergillus niger | |
| 1.14.13.23 | NADPH | - |
Aspergillus niger | |
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