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Literature summary extracted from

  • Groseclose, E.E.; Ribbons, D.W.
    3-Hydroxybenzoate 6-hydroxylase from Pseudomonas aeruginosa (1973), Biochem. Biophys. Res. Commun., 55, 897-903.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.24 0.16
-
 NADH
-
 Pseudomonas aeruginosa
1.14.13.24 0.8
-
 NADPH
-
 Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.24 85000
-
 sedimentation equilibrium measurement Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.24 Pseudomonas aeruginosa
-
-
-
1.14.13.24 Pseudomonas aeruginosa T1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.24 homogeneity Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.24 24
-
-
 Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.24 2,3-dihydroxybenzoate + NADH + O2 10.5% of the reaction with 3-hydroxybenzoate Pseudomonas aeruginosa 2,3,5-trihydroxybenzoate + NAD+ + H2O
-
 ?
1.14.13.24 2,3-dihydroxybenzoate + NADH + O2 10.5% of the reaction with 3-hydroxybenzoate Pseudomonas aeruginosa T1 2,3,5-trihydroxybenzoate + NAD+ + H2O
-
 ?
1.14.13.24 3,4-dihydroxybenzoate + NADH + O2 9% of the reaction with 3-hydroxybenzoate Pseudomonas aeruginosa 2,4,5-trihydroxybenzoate + NAD+ + H2O
-
 ?
1.14.13.24 3,4-dihydroxybenzoate + NADH + O2 9% of the reaction with 3-hydroxybenzoate Pseudomonas aeruginosa T1 2,4,5-trihydroxybenzoate + NAD+ + H2O
-
 ?
1.14.13.24 3,5-dihydroxybenzoate + NADH + O2 7% of the reaction with 3-hydroxybenzoate Pseudomonas aeruginosa 2,3,5-trihydroxybenzoate + NAD+ + H2O
-
 ?
1.14.13.24 3,5-dihydroxybenzoate + NADH + O2 7% of the reaction with 3-hydroxybenzoate Pseudomonas aeruginosa T1 2,3,5-trihydroxybenzoate + NAD+ + H2O
-
 ?
1.14.13.24 3-hydroxy-5-methylbenzoate + NADH + O2 31% of the reaction with 3-hydroxybenzoate Pseudomonas aeruginosa 2,5-dihydroxy-3-methylbenzoate + NAD+ + H2O
-
 ?
1.14.13.24 3-hydroxy-5-methylbenzoate + NADH + O2 31% of the reaction with 3-hydroxybenzoate Pseudomonas aeruginosa T1 2,5-dihydroxy-3-methylbenzoate + NAD+ + H2O
-
 ?
1.14.13.24 3-hydroxybenzoate + NADH + O2
-
 Pseudomonas aeruginosa 2,5-dihydroxybenzoate + NAD+ + H2O
-
 ?
1.14.13.24 3-hydroxybenzoate + NADH + O2
-
 Pseudomonas aeruginosa T1 2,5-dihydroxybenzoate + NAD+ + H2O
-
 ?
1.14.13.24 3-hydroxybenzoate + NADPH + H+ + O2
-
 Pseudomonas aeruginosa 2,5-dihydroxybenzoate + NADP+ + H2O
-
 r
1.14.13.24 4-fluoro-3-hydroxybenzoate + NADH + O2 36% of the reaction with 3-hydroxybenzoate Pseudomonas aeruginosa 4-fluoro-2,5-dihydroxybenzoate + NAD+ + H2O
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.24 FAD flavoprotein Pseudomonas aeruginosa
1.14.13.24 FAD 0.9 mol of FAD per mol of enzyme Pseudomonas aeruginosa
1.14.13.24 NADH
-
 Pseudomonas aeruginosa
1.14.13.24 NADPH can act instead of NADH, more slowly Pseudomonas aeruginosa