EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.13.25 | expression of His-tagged triple mutant G10A/G13Q/G16A and G13Q mutant in Escherichia coli | Methylococcus capsulatus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.13.25 | G10A/G13Q/G16A | reduced activity | Methylococcus capsulatus |
1.14.13.25 | G10A/G13Q/G16A | His-tagged protein B of sMMO, triple mutant is resistant to degradation in contrast to the wild-type, N-terminus is responsible for unusual mobility in size exclusion chromatography and proteolytic sensitivity of protein B | Methylococcus capsulatus |
1.14.13.25 | G13Q | reduced activity | Methylococcus capsulatus |
1.14.13.25 | G13Q | sMMO, alteration of a cleavage site in component protein B | Methylococcus capsulatus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.13.25 | additional information | - |
protein B shows unusual behaviour in gel filtration | Methylococcus capsulatus |
1.14.13.25 | 15850 | - |
component protein B of sMMO, mass spectrometry | Methylococcus capsulatus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.13.25 | Methylococcus capsulatus | - |
Bath | - |
1.14.13.25 | Methylococcus capsulatus | - |
enzyme form sMMO | - |
1.14.13.25 | Methylococcus capsulatus Bath | - |
Bath | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.13.25 | sMMO protein B wild-type and mutants recombinant from Escherichia coli by affinity chromatography, high salt concentration increases the binding stability between protein B and hydroxylase of sMMO | Methylococcus capsulatus |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.14.13.25 | methane + NAD(P)H + H+ + O2 = methanol + NAD(P)+ + H2O | structural model for component protein B of sMMO | Methylococcus capsulatus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.13.25 | 1.66 | - |
sMMO protein B triple mutant G10A/G13Q/G16A | Methylococcus capsulatus |
1.14.13.25 | 2.284 | - |
His-tagged sMMO protein B triple mutant G10A/G13Q/G16A | Methylococcus capsulatus |
1.14.13.25 | 3.99 | - |
sMMO protein B mutant G13Q | Methylococcus capsulatus |
1.14.13.25 | 5.09 | - |
sMMO, wild-type enzyme | Methylococcus capsulatus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.25 | methane + NAD(P)H + O2 | - |
Methylococcus capsulatus | methanol + NAD(P)+ + H2O | - |
? | |
1.14.13.25 | methane + NAD(P)H + O2 | - |
Methylococcus capsulatus Bath | methanol + NAD(P)+ + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.13.25 | MMO | - |
Methylococcus capsulatus |
1.14.13.25 | pMMO | particulate, membrane-bound enzyme form | Methylococcus capsulatus |
1.14.13.25 | sMMO | soluble, cytoplasmic enzyme form | Methylococcus capsulatus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.25 | NADH | - |
Methylococcus capsulatus |