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Literature summary extracted from

  • Brandstetter, H.; Whittington, D.A.; Lippard, S.J.; Frederick, C.A.
    Mutational and structural analyses of the regulatory protein B of soluble methane monooxygenase from Methylococcus capsulatus (Bath) (1999), Chem. Biol., 6, 441-449.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.25 expression of His-tagged triple mutant G10A/G13Q/G16A and G13Q mutant in Escherichia coli Methylococcus capsulatus

Protein Variants

EC Number Protein Variants Comment Organism
1.14.13.25 G10A/G13Q/G16A reduced activity Methylococcus capsulatus
1.14.13.25 G10A/G13Q/G16A His-tagged protein B of sMMO, triple mutant is resistant to degradation in contrast to the wild-type, N-terminus is responsible for unusual mobility in size exclusion chromatography and proteolytic sensitivity of protein B Methylococcus capsulatus
1.14.13.25 G13Q reduced activity Methylococcus capsulatus
1.14.13.25 G13Q sMMO, alteration of a cleavage site in component protein B Methylococcus capsulatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.25 additional information
-
protein B shows unusual behaviour in gel filtration Methylococcus capsulatus
1.14.13.25 15850
-
component protein B of sMMO, mass spectrometry Methylococcus capsulatus

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.25 Methylococcus capsulatus
-
Bath
-
1.14.13.25 Methylococcus capsulatus
-
enzyme form sMMO
-
1.14.13.25 Methylococcus capsulatus Bath
-
Bath
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.25 sMMO protein B wild-type and mutants recombinant from Escherichia coli by affinity chromatography, high salt concentration increases the binding stability between protein B and hydroxylase of sMMO Methylococcus capsulatus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.13.25 methane + NAD(P)H + H+ + O2 = methanol + NAD(P)+ + H2O structural model for component protein B of sMMO Methylococcus capsulatus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.25 1.66
-
sMMO protein B triple mutant G10A/G13Q/G16A Methylococcus capsulatus
1.14.13.25 2.284
-
His-tagged sMMO protein B triple mutant G10A/G13Q/G16A Methylococcus capsulatus
1.14.13.25 3.99
-
sMMO protein B mutant G13Q Methylococcus capsulatus
1.14.13.25 5.09
-
sMMO, wild-type enzyme Methylococcus capsulatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.25 methane + NAD(P)H + O2
-
Methylococcus capsulatus methanol + NAD(P)+ + H2O
-
?
1.14.13.25 methane + NAD(P)H + O2
-
Methylococcus capsulatus Bath methanol + NAD(P)+ + H2O
-
?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.25 MMO
-
Methylococcus capsulatus
1.14.13.25 pMMO particulate, membrane-bound enzyme form Methylococcus capsulatus
1.14.13.25 sMMO soluble, cytoplasmic enzyme form Methylococcus capsulatus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.25 NADH
-
Methylococcus capsulatus