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Literature summary extracted from
- Metal ions and chloramphenicol inhibition of soluble methane monooxygenase from Methylosinus trichosporium OB3b (1996), Appl. Microbiol. Biotechnol., 45, 744-749.
No PubMed abstract available
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.13.25 | transcription of sMMO is repressed at Cu2+ concentration above 0.00086 mM per g dry cell weight | Methylosinus trichosporium |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.25 | ammonium chloride | slightly | Methylosinus trichosporium |  |
| 1.14.13.25 | chloramphenicol | sMMO | Methylosinus trichosporium | |
| 1.14.13.25 | Cu+ | soluble enzyme form sMMO | Methylosinus trichosporium | |
| 1.14.13.25 | Cu2+ | soluble enzyme form, sMMO | Methylosinus trichosporium | |
| 1.14.13.25 | Fe3+ | slightly, soluble enzyme form sMMO | Methylosinus trichosporium | |
| 1.14.13.25 | methylamine | slightly | Methylosinus trichosporium | |
| 1.14.13.25 | Ni2+ | sMMO | Methylosinus trichosporium | |
| 1.14.13.25 | Zn2+ | sMMO | Methylosinus trichosporium | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.13.25 | cytoplasm | enzyme from cells grown under conditions of low copper availability | Methylosinus trichosporium | 5737 | - |
| 1.14.13.25 | cytoplasm | cytoplasmatic, soluble enzyme form termed sMMO | Methylosinus trichosporium | 5737 | - |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.13.25 | Methylosinus trichosporium | - |
- |
- |
| 1.14.13.25 | Methylosinus trichosporium | - |
enzyme form sMMO | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.13.25 | sMMO | Methylosinus trichosporium |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.13.25 | methane + NAD(P)H + O2 | - |
Methylosinus trichosporium | methanol + NAD(P)+ + H2O | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.13.25 | MMO | - |
Methylosinus trichosporium |
| 1.14.13.25 | pMMO | particulate, membrane-bound enzyme form | Methylosinus trichosporium |
| 1.14.13.25 | sMMO | soluble, cytoplasmic enzyme form | Methylosinus trichosporium |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.25 | NADH | - |
Methylosinus trichosporium | |
| 1.14.13.25 | NADPH | - |
Methylosinus trichosporium | |
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