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Literature summary extracted from
- Protein B of soluble methane monooxygenase from Methylococcus capsulatus (Bath). A novel regulatory protein of enzyme activity (1985), J. Biol. Chem., 260, 15795-15801.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.14.13.25 | protein B stable to successive freezing and thawing | Methylococcus capsulatus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.13.25 | additional information | - |
additional information | - |
Methylococcus capsulatus |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.25 | Ni2+ | protein B contains 0.04 mol Ni2+ per mol protein | Methylococcus capsulatus |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.13.25 | Methylococcus capsulatus | - |
Bath | - |
| 1.14.13.25 | Methylococcus capsulatus Bath | - |
Bath | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.13.25 | protein B | Methylococcus capsulatus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.25 | 7.3 | - |
protein B | Methylococcus capsulatus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.13.25 | methane + NAD(P)H + O2 | - |
Methylococcus capsulatus | methanol + NAD(P)+ + H2O | - |
? | |
| 1.14.13.25 | methane + NAD(P)H + O2 | - |
Methylococcus capsulatus Bath | methanol + NAD(P)+ + H2O | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.25 | 45 | - |
assay at, protein B | Methylococcus capsulatus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.25 | 45 | - |
protein B, half-life: 10.2 min | Methylococcus capsulatus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.25 | 6 | 7 | protein B, stable, rapid loss of activity above and below | Methylococcus capsulatus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.25 | NADH | - |
Methylococcus capsulatus | |
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Release 2023.1 (January 2023)
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