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Literature summary extracted from

  • Pilkington, S.J.; Dalton, H.
    Purification and characterization of the soluble methane monooxygenase from Methylosinus sporium 5 demonstrates the highly conserved nature of this enzyme in methanotrophs (1991), FEMS Microbiol. Lett., 78, 103-108.
No PubMed abstract available

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.13.25 cytoplasm
-
 Methylosinus sporium 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.25 Iron 2.1 mol Fe per mol enzyme Methylosinus sporium
1.14.13.25 Iron 1 mol Fe per mol enzyme Methylosinus sporium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.25 additional information
-
 see under subunits: molecular weights of the subunits of components Methylosinus sporium
1.14.13.25 additional information
-
 enzyme system consists of 3 protein components A, B, C Methylosinus sporium
1.14.13.25 20000
-
 component A: 2 * 56000 alpha + 2 * 40000 beta + 2 * 20000 gamma, SDS-PAGE Methylosinus sporium
1.14.13.25 40000
-
 component C acceptor reductase Methylosinus sporium
1.14.13.25 56000
-
 component A: 2 * 56000 alpha + 2 * 40000 beta + 2 * 20000 gamma, SDS-PAGE Methylosinus sporium
1.14.13.25 220000
-
 component A: hydroxylase, gel filtration Methylosinus sporium

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.25 Methylosinus sporium
-
-
-
1.14.13.25 Methylosinus sporium 5
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.25 protein A and C Methylosinus sporium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.25 0.185
-
 component A Methylosinus sporium
1.14.13.25 17.5
-
 component C Methylosinus sporium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.25 methane + NAD(P)H + O2
-
 Methylosinus sporium methanol + NAD(P)+ + H2O
-
 ?
1.14.13.25 methane + NAD(P)H + O2
-
 Methylosinus sporium 5 methanol + NAD(P)+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.13.25 ? component A: 2 * 56000 alpha + 2 * 40000 beta + 2 * 20000 gamma, SDS-PAGE Methylosinus sporium
1.14.13.25 More see under molecular weight for the size of the protein components Methylosinus sporium
1.14.13.25 More enzyme system consists of 3 protein components A, B, C Methylosinus sporium

Synonyms

EC Number Synonyms Comment Organism
1.14.13.25 MMO
-
 Methylosinus sporium

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.25 FAD protein C, reductase component: contains 1 mol FAD per mol protein Methylosinus sporium
1.14.13.25 NADH
-
 Methylosinus sporium