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Literature summary extracted from

  • Tsuji, H.; Ogawa, T.; Bando, N.; Sasaoka, K.
    A unique enzyme catalyzing the formation of 4-hydroxyaniline from 4-amino-benzoic acid in Agaricus bisporus (1985), Biochem. Biophys. Res. Commun., 130, 633-639.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.27 Cu2+ severe inhibition at 1 mM Agaricus bisporus
1.14.13.27 Fe2+ slight inhibition at 1 mM Agaricus bisporus
1.14.13.27 Hg2+ complete inhibition at 0.1 mM Agaricus bisporus
1.14.13.27 p-chloromercuribenzoate complete inhibition at 0.1 mM Agaricus bisporus

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.27 Agaricus bisporus
-
 mushroom
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.27
-
 Agaricus bisporus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.13.27 fruit body
-
 Agaricus bisporus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.27 0.5
-
-
 Agaricus bisporus

Storage Stability

EC Number Storage Stability Organism
1.14.13.27 -20°C, 10 mM potassium phosphate buffer, pH 7.0, 20% glycerol, 6 months, stable Agaricus bisporus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.27 4-aminobenzoate + NAD(P)H + O2
-
 Agaricus bisporus 4-hydroxyaniline + NAD(P)+ + H2O + CO2
-
 ?
1.14.13.27 4-aminosalicylate + NAD(P)H + O2
-
 Agaricus bisporus 3,4-dihydroxyaniline + NAD(P)+ + H2O2 + CO2
-
 ?
1.14.13.27 anthranilate + NAD(P)H + O2
-
 Agaricus bisporus 2-hydroxyaniline + NAD(P)+ + H2O2 + CO2
-
 ?
1.14.13.27 additional information not: aniline Agaricus bisporus ?
-
 ?
1.14.13.27 additional information not: 4-aminobenzyl-alcohol Agaricus bisporus ?
-
 ?
1.14.13.27 additional information not: benzoic acid Agaricus bisporus ?
-
 ?
1.14.13.27 additional information not: salicylate Agaricus bisporus ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.27 7
-
-
 Agaricus bisporus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.27 FAD flavoprotein Agaricus bisporus
1.14.13.27 NADH
-
 Agaricus bisporus
1.14.13.27 NADPH 68% of the activity with NADH Agaricus bisporus