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Literature summary extracted from

  • Tsuji, H.; Ogawa, T.; Bando, N.; Sasaoka, K.
    Purification and properties of 4-aminobenzoate hydroxylase, a new monooxygenase from Agaricus bisporus (1986), J. Biol. Chem., 261, 13203-13209.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.27 AgNO3 93% inactivation at 0.1 mM Agaricus bisporus
1.14.13.27 Br- 37% inactivation at 0.1 M Agaricus bisporus
1.14.13.27 Cl- 30% inactivation at 0.1 M Agaricus bisporus
1.14.13.27 CuSO4 91% inactivation at 0.1 mM Agaricus bisporus
1.14.13.27 F- 20% inactivation at 0.1 M Agaricus bisporus
1.14.13.27 HgCl2 92% inactivation at 0.1 mM Agaricus bisporus
1.14.13.27 I- 62% inactivation at 0.1 M Agaricus bisporus
1.14.13.27 NO3- 45% inactivation at 0.1 M Agaricus bisporus
1.14.13.27 p-chloromercuribenzoate 94% inactivation at 0.1 mM Agaricus bisporus
1.14.13.27 SCN- 60% inactivation at 0.1 M Agaricus bisporus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.27 0.0136
-
 NADH
-
 Agaricus bisporus
1.14.13.27 0.0204
-
 4-Aminobenzoate
-
 Agaricus bisporus
1.14.13.27 0.133
-
 NADPH
-
 Agaricus bisporus
1.14.13.27 0.2
-
 O2
-
 Agaricus bisporus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.27 49000
-
 gel filtration Agaricus bisporus
1.14.13.27 50000
-
 SDS-PAGE Agaricus bisporus

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.27 Agaricus bisporus
-
 mushroom
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.27 homogeneity Agaricus bisporus

Storage Stability

EC Number Storage Stability Organism
1.14.13.27 -20°C, 50 mM potassium phosphate buffer, pH 7.0, 20% glycerol, 1 mM 2-mercaptoethanol, 0.01 mM FAD, stable for 1 month Agaricus bisporus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.27 2-amino-5-chlorobenzoate + NAD(P)H + O2
-
 Agaricus bisporus 4-chloro-2-hydroxyaniline + NAD(P)+ + H2O2 + CO2 H2O2 formed simultaneously with hydroxylation ?
1.14.13.27 3,4-diaminobenzoate + NAD(P)H + O2 17.3% of the reaction with 4-aminobenzoate Agaricus bisporus 2-amino-4-hydroxyaniline + NAD(P)+ + H2O2 + CO2 H2O2 formed simultaneously with hydroxylation ?
1.14.13.27 4-amino-2-chlorobenzoate + NAD(P)H + O2
-
 Agaricus bisporus 3-chloro-4-hydroxyaniline + NAD(P)+ + H2O2 + CO2 H2O2 formed simultaneously with hydroxylation ?
1.14.13.27 4-aminobenzoate + NAD(P)H + O2
-
 Agaricus bisporus 4-hydroxyaniline + NAD(P)+ + H2O + CO2
-
 ?
1.14.13.27 4-aminobenzoate + NADH + H+ + O2
-
 Agaricus bisporus 4-hydroxyaniline + NAD+ + H2O + CO2
-
 r
1.14.13.27 4-aminosalicylate + NAD(P)H + O2
-
 Agaricus bisporus 3,4-dihydroxyaniline + NAD(P)+ + H2O2 + CO2 H2O2 formed simultaneously with hydroxylation ?
1.14.13.27 4-hydroxybenzoate + NAD(P)H + O2 8.4% of the reaction with 4-aminobenzoate Agaricus bisporus quinol + NAD(P)+ + H2O2 + CO2 H2O2 formed simultaneously with hydroxylation ?
1.14.13.27 anthranilate + NAD(P)H + O2
-
 Agaricus bisporus 2-hydroxyaniline + NAD(P)+ + H2O2 + CO2 H2O2 formed simultaneously with hydroxylation ?
1.14.13.27 additional information several aromatic compounds stimulate NADH oxidation, but no hydroxylated product is formed, all of the O2 consumed is converted to H2O2 Agaricus bisporus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.13.27 monomer 1 * 49000, SDS-PAGE Agaricus bisporus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.27 40
-
-
 Agaricus bisporus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.13.27 30
-
 60 min, little loss of activity in presence of 0.1 mM FAD and 0.02% bovine serum albumin Agaricus bisporus
1.14.13.27 35
-
 10 min, 50 mM potassium phosphate buffer, pH 7.0, 0.1 mM FAD, 0.02% bovine serum albumin, stable Agaricus bisporus
1.14.13.27 40
-
 10 min, 50 mM potassium phosphate buffer, pH 7.0, 0.1 mM FAD, 0.02% bovine serum albumin, about 70% loss of activity Agaricus bisporus
1.14.13.27 45
-
 10 min, 50 mM potassium phosphate buffer, pH 7.0, 0.1 mM FAD, 0.02% bovine serum albumin, 97% loss of activity Agaricus bisporus
1.14.13.27 50
-
 10 min, 50 mM potassium phosphate buffer, pH 7.0, 0.1 mM FAD, 0.02% bovine serum albumin, 98% loss of activity Agaricus bisporus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.27 6 7 NADPH Agaricus bisporus
1.14.13.27 6.5 8 NADH Agaricus bisporus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.14.13.27 6 8 stable Agaricus bisporus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.27 FAD flavoprotein Agaricus bisporus
1.14.13.27 FAD 0.91 mol of FAD per mol of enzyme Agaricus bisporus
1.14.13.27 NADH
-
 Agaricus bisporus
1.14.13.27 NADPH 68% of the activity with NADH Agaricus bisporus
1.14.13.27 NADPH 62% of the activity with NADH Agaricus bisporus