Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Zeyer, J.; Kocher, H.P.; Timmis, K.N.
    Influence of para-substituents on the oxidative metabolism of o-nitrophenols by Pseudomonas putida B2 (1986), Appl. Environ. Microbiol., 52, 334-339.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.31 Cu2+
-
 Pseudomonas putida

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.13.31 soluble
-
 Pseudomonas putida
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.31 Ca2+ 30% stimulation at 4 mM Pseudomonas putida
1.14.13.31 Mg2+ 50% stimulation at 4 mM, EDTA reverses stimulation Pseudomonas putida
1.14.13.31 Mn2+ 50% stimulation at 4 mM Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.31 2-nitrophenol + NADPH + O2 Pseudomonas putida involved in metabolism of nitro-aromatic compounds by a strain of Pseudomonas putida ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.31 Pseudomonas putida
-
-
-
1.14.13.31 Pseudomonas putida B2
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.31 12
-
 with 4-chloro-2-nitrophenol as substrate Pseudomonas putida
1.14.13.31 50
-
 with 4-methyl-2-nitrophenol as substrate Pseudomonas putida
1.14.13.31 60
-
 with 2-nitrophenol as substrate Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.31 2-nitrophenol + NADPH + H+ + O2
-
 Pseudomonas putida catechol + nitrite + NADP+ + H2O
-
 ?
1.14.13.31 2-nitrophenol + NADPH + H+ + O2
-
 Pseudomonas putida B2 catechol + nitrite + NADP+ + H2O
-
 ?
1.14.13.31 2-nitrophenol + NADPH + O2 involved in metabolism of nitro-aromatic compounds by a strain of Pseudomonas putida Pseudomonas putida ?
-
 ?
1.14.13.31 4-chloro-2-nitrophenol + NADPH + O2
-
 Pseudomonas putida 4-chlorocatechol + NADP+ + H2O
-
 ?
1.14.13.31 4-chloro-2-nitrophenol + NADPH + O2
-
 Pseudomonas putida B2 4-chlorocatechol + NADP+ + H2O
-
 ?
1.14.13.31 4-formyl-2-nitrophenol + NADPH + O2 weak activity Pseudomonas putida 4-formyl-1,2-benzenediol + NADP+ + H2O
-
 ?
1.14.13.31 4-formyl-2-nitrophenol + NADPH + O2 weak activity Pseudomonas putida B2 4-formyl-1,2-benzenediol + NADP+ + H2O
-
 ?
1.14.13.31 4-methyl-2-nitrophenol + NADPH + O2
-
 Pseudomonas putida 4-methylcatechol + NADP+ + H2O
-
 ?
1.14.13.31 4-methyl-2-nitrophenol + NADPH + O2
-
 Pseudomonas putida B2 4-methylcatechol + NADP+ + H2O
-
 ?
1.14.13.31 additional information not: 4-carboxy-2-nitrophenol, 4-nitro-2-nitrophenol Pseudomonas putida ?
-
 ?
1.14.13.31 additional information not: 4-carboxy-2-nitrophenol, 4-nitro-2-nitrophenol Pseudomonas putida B2 ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.31 7.5
-
 assay at Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.31 NADPH
-
 Pseudomonas putida