EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.35 | 1,10-phenanthroline | 46% inhibition at 0.5 mM , 60% inhibition at 1 mM, 80% inhibition at 3 mM, anthranilic acid protects the enzyme from inhibition | Aspergillus niger | |
1.14.13.35 | 2,2'-dipyridyl | 27% inhibition at 0.5 mM, 41% inhibition at 1 mM | Aspergillus niger | |
1.14.13.35 | 8-hydroxyquinoline | 23% inhibition at 0.5 mM, 37% inhibition at 1 mM | Aspergillus niger | |
1.14.13.35 | EDTA | 7% inhibition at 1 mM | Aspergillus niger | |
1.14.13.35 | additional information | reversal of 1,10-phenanthroline inhibition by ferric-EDTA, ferrous-EDTA, ferric citrate and cytochrome c | Aspergillus niger | |
1.14.13.35 | Salicylaldoxime | 40% inhibition at 0.5 mM, 52% inhibition at 1 mM | Aspergillus niger |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.35 | Iron | the partially purified enzyme is not activated by any metal ion but a considerable decrease in anthranilate hydroxylase activity occurs when the organism is grown on a medium deprived of iron | Aspergillus niger | |
1.14.13.35 | Iron | enzyme-bound iron, participation of Fe in reaction, omission of Fe in the growth medium yields inactive preparation | Aspergillus niger |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.13.35 | Aspergillus niger | - |
UBC 814 | - |
1.14.13.35 | Aspergillus niger UBC 814 | - |
UBC 814 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.13.35 | partial, using centrifugation, protamine sulfate treatment, treatment with diethylaminoethyl-cellulose and filtration through a Buchner funnel | Aspergillus niger |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.13.35 | 0.28 | - |
organism grown on iron-deficient medium, no addition of other constituents | Aspergillus niger |
1.14.13.35 | 2.2 | - |
organism grown on iron-deficient medium, addition of 1 mM ferric citrate, preincubation time: 5 min | Aspergillus niger |
1.14.13.35 | 4.2 | - |
organism grown on iron-deficient medium, addition of 1 mM ferric citrate, preincubation time: 10 min | Aspergillus niger |
1.14.13.35 | 4.6 | - |
organism grown on iron-deficient medium, addition of 1 mM ferric-EDTA | Aspergillus niger |
1.14.13.35 | 4.9 | - |
organism grown on iron-deficient medium, addition of 1 mM ferric citrate, preincubation time: 15, 20 or 30 min | Aspergillus niger |
1.14.13.35 | 5.6 | - |
organism grown on standard iron-sufficient medium | Aspergillus niger |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.35 | anthranilate + NADPH + O2 | - |
Aspergillus niger | 2,3-dihydroxybenzoate + NADP+ + NH3 | - |
? | |
1.14.13.35 | anthranilate + NADPH + O2 | - |
Aspergillus niger UBC 814 | 2,3-dihydroxybenzoate + NADP+ + NH3 | - |
? |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.13.35 | 8.2 | - |
assay at | Aspergillus niger |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.35 | NADPH | - |
Aspergillus niger |